You are viewing an incomplete version of our website. Please click to reload the website as full version.

Integrin, alpha 6 (ITGA6) (Isoform 6B), (N-Term) antibody

Details for Product No. ABIN112373, Supplier: Login to see
Antigen
  • inta6
  • ITGA6
  • im:6897425
  • itga6
  • si:dkey-32o22.1
  • wu:fb80g02
  • LOC100221694
  • 5033401O05Rik
  • AI115430
  • Cd49f
  • VLA-6
  • CD49f
  • ITGA6B
Epitope
N-Term, Isoform 6B
18
14
13
12
9
7
7
5
5
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
274
96
35
18
17
12
8
6
2
2
2
2
1
1
1
Host
Mouse
105
100
99
2
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
40
32
19
5
5
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
184
103
74
51
46
42
28
18
11
8
7
7
5
3
2
2
2
1
Supplier
Login to see
Supplier Product No.
Login to see
Request

Showcase your results, aid the scientific community, and receive a full refund.

Contribute a validation

Learn more

Immunogen 6B4 is a mouse monoclonal IgG1, k antibody derived by fusion of SP2/0 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a synthetic peptide corresponding to a 34 amino acid stretch in the cytoplasmic domain of integrin a6B including an appending N-terminal Cysteine coupled to keyhole limpet hemocyanin.
Sequence CSRYDDSVPR YHAVRIRKEE REIKDEKYID NLEKK
Clone 6B4
Isotype IgG1
Specificity It recognizes specifically the cytoplasmic domain of integrin subunit alpha-6B. 6B4 stains the basement membrane zone of ducts and acini of salivary gland, kidney tubules, epithelium of colonic crypts and weakly stains sweat glands and sebaceous glands. A broad species reactivity is expected because of the conserved nature of the epitope.
Purification Purified
Alternative Name CD49f / ITGA6 (ITGA6 Antibody Abstract)
Background Integrins are a family of heterodimeric membrane glycoproteins consisting of non-covalently associated a and b subunits. More than 18 a and 8 b subunits with numerous splice variant isoforms have been identified in mammals. In general, integrins function as receptors for extracellular matrix proteins. Certain integrins can also bind to soluble ligands or to counter-receptors on adjacent cells, such as the intracellular adhesion molecules (ICAMs), resulting in aggregation of cells. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. For integrin subunits a3 and a6, two cytoplasmic variants, A and B, have been identified.Synonyms: CD49 antigen-like family member F, Integrin alpha-6, VLA-6, VLA6
Gene ID 3655
NCBI Accession NP_000201
UniProt P23229
Pathways
Application Notes Suitable for Immunoblotting, Immunocytochemistry, Immunohistochemistry on frozensections. Recommended dilutions: 1/50-1/100 for Flow cytometry, and for Immunohistochemistrywith avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and1/100-1/500 for Immunoblotting applications.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the lyophilized antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer. When reconstituted the antibody is stable for one month at 2-8 °C.
Background publications Hogervorst, Admiraal, Niessen et al.: "Biochemical characterization and tissue distribution of the A and B variants of the integrin alpha 6 subunit." in: The Journal of cell biology, Vol. 121, Issue 1, pp. 179-91, 1993 (PubMed).