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Cardiotin antibody

Reactivity: Human, Pig, Monkey, Cat, Rat, Dog, Xenopus laevis, Goat, Hamster, Mouse, Zebrafish (Danio rerio) WB, IHC (fro), IHC (p) Host: Mouse Monoclonal R2G unconjugated
Catalog No. ABIN112391
  • Target
    Cardiotin
    Reactivity
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Human, Pig, Monkey, Cat, Rat, Dog, Xenopus laevis, Goat, Hamster, Mouse, Zebrafish (Danio rerio)
    Host
    • 4
    Mouse
    Clonality
    • 4
    Monoclonal
    Conjugate
    • 4
    Un-conjugated
    Application
    • 4
    • 4
    • 3
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Specificity
    R2G recognizes the 300 kDa cardiotin protein complex and its 100 kDa and 60 kDa subunits. This antibody reacts with Canine, Feline, Goat, Hamster, Human, Monkey, Mouse, Rabbit, Rat, Xenopus, Zebrafish tissues.
    Purification
    Purified
    Immunogen
    Total protein extract of Chicken gizzard.
    Clone
    R2G
    Isotype
    IgM
  • Application Notes
    This antibody is suitable for Immunohistochemistry on frozen and paraffin-embeddedtissue and Immunoblotting. Recommended dilutions: 1/25-1/100 for immunohistochemistry with avidinbiotinylatedhorseradish peroxidase complex (ABC)as detection reagent.1/25-1/500 for Immunoblotting applications.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Buffer
    PBS with 0.09 % Sodium Azide as preservative
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the lyophilized antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer. When reconstituted the antibody is stable for one month at 2-8 °C.
  • Target
    Cardiotin
    Background
    Cardiotin is a high molecular weight protein complex (300 kDa) located in the mitochondria of cardiomyocytes and skeletal muscle. The cardiotin structure exists of subunits of 60 kDa and 100 kDa, probably in a tetrameric configuration. Both subunits contain the same amino-terminal 14 amino-acid sequence, showing high homology to human skeletal muscle α-actinin. During cardiac contractile dysfunction and myocard cell differentiation, the cardiotin distribution is affected. Compared to other structural proteins, cardiotin is one of the first to respond to insults (ischemia, fibrillation) that influence the functional status of cardiomyocytes.
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