Lactoferrin antibody (HRP)

Catalog No. ABIN113035

Product Details anti-Lactoferrin Antibody (HRP)

Target: Lactoferrin (LTF) (Lactotransferrin (LTF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Lactoferrin antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purification: Immunoaffinity chromatography using antigens coupled to agarose beads.

Immunogen: Lactoferrin purified from human colostrum.

Application Details

Application Notes: Immunohistochemistry (1: 500 - 1: 5,000) on tissue sections.1: 5,000 - 1: 100,000 for ELISA and Western blotting with chromogenic substrates and1: 10,000 - 1: 200,000 for Western blotting with ECL substrates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 1.5 mL distilled water. Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature.

Concentration: 0.8 mg/mL (prior to lyophilization)

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.6, containing 15 mg/mL BSA (IgG and Protease free)

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store at 2 - 8 °C.

Target Details for Lactoferrin

Target: Lactoferrin (LTF) (Lactotransferrin (LTF))

Alternative Name: Lactotransferrin (LTF Products)

Synonyms: LTF antibody, GIG12 antibody, HLF2 antibody, LF antibody, Lf antibody, Csp82 antibody, MMS10R antibody, Ms10r antibody, PLF antibody, TFEW antibody, conalbumin antibody, lactotransferrin antibody, transferrin antibody, transferrin (ovotransferrin) antibody, LTF antibody, CpipJ_CPIJ000114 antibody, TF antibody, Ltf antibody

Background: Lactoferrin belongs to a family of iron-binding proteins that modulate iron metabolism, hemopoiesis, and immunologic reactions, together with transferrin and melanoma tumor antigen p97. They are evolutionary products of gene duplication and all 3 are encoded by genes on 3q. Lactoferrin is an iron binding glycoprotein with an approximate molecular weight of 80 kDa. The protein has two iron binding domains each housing one Fe3+ and the synergistic CO32- ion. The crystal structure form of human lactoferrin at 2.2A resolution exhibits 5330 protein atoms, 2Fe2+, 2CO32- and 98 carbohydrate atoms. Lactoferrin is absorbed from intestine by apical side of the membrane and localized to the nuclei. Intravenous infusion of lactoferrin is protective against lethal doses of E coli and induce bacterimia by a mechanism that downregulates neutrophil TNF alfa secretion. Recombinant human lactoferrin (rhLF), expressed and extracted from rice seed, is being evaluated for use as a dietary supplement to treat iron deficiency and/or iron deficiency induced anemia. Lactoferrin has been shown to have a role in the immune system and in early development of the embryo. A specific receptor for lactoferrin binding has been implicated in the human fetal intestine. Early embryonic localisation of lactoferrin by IHC has suggested its presence in various tissues including intestinal epitheliuem, kiney, and various regions of the brain.Synonyms: EC=3.4.21, LF, LTF, Lactoferrin, Talalactoferrin

Gene ID: 9606

UniProt: P02788

Pathways: Transition Metal Ion Homeostasis