This antibody reacts with natural and recombinant human EGF. Does not cross react with other Human cytokines tested such as IL-1 beta, IL-8, MCAF, TGF-beta and SAA.
Cross-Reactivity (Details)
Species reactivity (tested):Human.
Purification
Affinity Chromatograpghy on Protein G.
Immunogen
Purified recombinant Human EGF, 6kDa, 53 amino acid residues
ELISA: This antibody can be used as a Capture antibody in sandwich ELISA applications forHuman EGF detection in combination with a monoclonal tracer/detection antibody. Suggested Capture Coating Dose: 0.3 μg/well, Substrate: TMB. If the above suggestedconditions are followed approximately 20 pg/mL of EGF in serum/plasma or 15 pg/mL ofEGF in cell medium/urine can be detected with an assay range of 0-1000 pg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore with double distillated water is recommended to adjust the final concentration to 1.00 mg/mL
Concentration
1.00 mg/mL
Buffer
0.01 M PBS, pH 7.2 without preservatives
Preservative
Without preservative
Handling Advice
Avoid repeated freezing and thawing.
Storage
-20 °C
Storage Comment
Store the antibody at -20 °C. Can be shipped at 2 - 8 °C.
Epidermal growth factor (EGF) has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF exerts its actions by binding to the EGFR, a 170 kDa protein. Epidermal growth factor (EGF) is a key growth factor regulating cell survival. Through its binding to cell surface receptors, EGF activates an extensive network of signal transduction pathways that include activation of the PI3K/AKT, RAS/ERK and JAK/STAT pathways. Because of its key role in driving the proliferation of cells, EGFR is a target of several anti-cancer drugs currently in development.Synonyms: Epidermal growth factor, HOMG4, Pro-epidermal growth factor, URG, Urogastrone