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Keratin 17 (KRT17) antibody

Details for Product No. ABIN114619, Supplier: Log in to see
Antigen
  • K17
  • Ka17
  • Krt1-17
  • krt16
  • KRT17
  • PC
  • PC2
  • PCHC1
  • si:dkeyp-113d7.7
Reactivity
Human, Rat (Rattus)
275
156
78
78
55
50
24
5
2
1
1
1
Host
Mouse
188
101
5
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
10
8
7
7
5
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
2
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
214
144
113
110
106
101
88
80
55
12
3
2
2
2
2
Supplier
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Immunogen A cytoskeletal preparation from rat colon
Clone Ks17-E3
Isotype IgG2b
Specificity Clone E3 reacts with Cytokeratin 17 in basal layers of pseudo-stratified and transitional epithelia.
Cross-Reactivity (Details) Species reactivity (tested):Human, Rat.
Purification Purified
Alternative Name Cytokeratin 17 (KRT17 Antibody Abstract)
Background Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immuno-histochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.Synonyms: CK17, Cytokeratin-17, K17, KRT17, Keratin 17, Keratin type I cytoskeletal 17, Keratin-17
Molecular Weight 46 kDa
Gene ID 3872
NCBI Accession NP_000413
UniProt Q04695
Research Area Signaling, Cytoskeleton, Cytokeratins, Extracellular Matrix
Application Notes Immunoblotting (1: 100 - 1: 1000). Immunocytochemistry. Immunohistochemistry on frozen and paraffin-embedded tissues (1: 25- 1: 200 forimmunohistochemistry with avidin-biotinylated horseradish peroxidase complec (ABC) asdetection regant). Flow cytometry (1: 25-1: 200)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer PBS, 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Litvinov, van Driel, van Rhijn, Bakker, van Krieken, Fleuren, Warnaar: "Expression of Ep-CAM in cervical squamous epithelia correlates with an increased proliferation and the disappearance of markers for terminal differentiation." in: The American journal of pathology, Vol. 148, Issue 3, pp. 865-75, 1997 (PubMed).

Smedts, Ramaekers, Link, Lauerova, Troyanovsky, Schijf, Vooijs: "Detection of keratin subtypes in routinely processed cervical tissue: implications for tumour classification and the study of cervix cancer aetiology." in: Virchows Archiv : an international journal of pathology, Vol. 425, Issue 2, pp. 145-55, 1994 (PubMed).

Smedts, Ramaekers, Troyanovsky, Pruszczynski, Link, Lane, Leigh, Schijf, Vooijs: "Keratin expression in cervical cancer." in: The American journal of pathology, Vol. 141, Issue 2, pp. 497-511, 1992 (PubMed).

Smedts, Ramaekers, Troyanovsky, Pruszczynski, Robben, Lane, Leigh, Plantema, Vooijs: "Basal-cell keratins in cervical reserve cells and a comparison to their expression in cervical intraepithelial neoplasia." in: The American journal of pathology, Vol. 140, Issue 3, pp. 601-12, 1992 (PubMed).

Wetzels, Schaafsma, Leigh, Lane, Troyanovsky, Wagenaar, Vooijs, Ramaekers: "Laminin and type VII collagen distribution in different types of human lung carcinoma: correlation with expression of keratins 14, 16, 17 and 18." in: Histopathology, Vol. 20, Issue 4, pp. 295-303, 1992 (PubMed).

Wetzels, Kuijpers, Lane, Leigh, Troyanovsky, Holland, van Haelst, Ramaekers: "Basal cell-specific and hyperproliferation-related keratins in human breast cancer." in: The American journal of pathology, Vol. 138, Issue 3, pp. 751-63, 1991 (PubMed).

Troyanovsky, Guelstein, Tchipysheva, Krutovskikh, Bannikov: "Patterns of expression of keratin 17 in human epithelia: dependency on cell position." in: Journal of cell science, Vol. 93 ( Pt 3), pp. 419-26, 1990 (PubMed).

Guelstein, Tchypysheva, Ermilova, Litvinova, Troyanovsky, Bannikov: "Monoclonal antibody mapping of keratins 8 and 17 and of vimentin in normal human mammary gland, benign tumors, dysplasias and breast cancer." in: International journal of cancer. Journal international du cancer, Vol. 42, Issue 2, pp. 147-53, 1988 (PubMed).