Keratin 19 (KRT19) antibody

Details for Product No. ABIN114797, Supplier: Log in to see
Antigen
  • k19
  • ck19
  • k1cs
  • krt9
  • krt15
  • MGC76282
  • MGC83069
  • KRT19
  • CK19
  • K19
  • K1CS
  • GK-19
  • Ka19
  • Krt1-19
  • AI663979
  • EndoC
  • Krt-1.19
  • keratin 19
  • KRT19
  • krt19
  • Krt19
Reactivity
Human
911
211
136
19
18
15
11
9
7
5
5
4
3
3
2
2
1
1
1
1
1
1
1
Host
Mouse
683
223
24
5
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
52
32
30
20
17
17
17
17
17
16
16
16
15
15
15
15
13
7
7
6
4
3
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
632
476
328
315
306
292
233
191
107
19
15
12
11
10
3
3
2
2
1
1
1
1
1
1
1
Options
Supplier
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Supplier Product No.
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Immunogen Human breast cancer cell line MCF-7
Clone Ks19-1 (A53-B-A2)
Isotype IgG2a
Specificity Specific for the rod domain (aa 312-335) of Human Cytokeratin 19. In Western blotting 40 kDa and 19 kDa bands are observed.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Affinity Chromatography on Protein G.
Alternative Name Cytokeratin 19 (KRT19 Antibody Abstract)
Background This protein is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Keratin 19 is not expressed in hepatocytes, therefore, antibody to keratin 19 is useful in the identification of liver metastasis. The degree of keratin 19 positivity in breast cancer distinguishes malignant from benign tumours. Keratin 19 is often co-expressed with keratin 7.Synonyms: CK19, Cytokeratin-19, K19, KRT19, Keratin type I cytoskeletal 19, Keratin-19
Gene ID 3880
NCBI Accession NP_002267
UniProt P08727
Research Area Signaling, Cytoskeleton, Cytokeratins, Extracellular Matrix
Application Notes ELISA. Western blot. Immunofluorescence. Immunohistochemistry on Paraffin Sections: 1/100-1/200 (Requires protein digestionpre-treatment of paraffin sections e. g. trypsin or pronase). Use appendix as Positive Control.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunohistochemistry (IHC) image for anti-Keratin 19 (KRT19) antibody (ABIN114797) Staining of paraffin embedded human appendix with mouse anti human cytokeratin 19 (AB...
Background publications Goletz, Hanisch, Karsten: "Novel alphaGalNAc containing glycans on cytokeratins are recognized invitro by galectins with type II carbohydrate recognition domains." in: Journal of cell science, Vol. 110 ( Pt 14), pp. 1585-96, 1997 (PubMed).

Kasper, Stosiek, Typlt, Karsten: "Histological evaluation of three new monoclonal anti-cytokeratin antibodies. 1. Normal tissues." in: European journal of cancer & clinical oncology, Vol. 23, Issue 2, pp. 137-47, 1988 (PubMed).

Karsten, Papsdorf, Roloff, Stolley, Abel, Walther, Weiss: "Monoclonal anti-cytokeratin antibody from a hybridoma clone generated by electrofusion." in: European journal of cancer & clinical oncology, Vol. 21, Issue 6, pp. 733-40, 1985 (PubMed).