Thrombospondin 1 antibody (THBS1)

Details for Product anti-THBS1 Antibody No. ABIN115004, Supplier: Log in to see
Antigen
  • TMTSP
  • UNQ3010
  • THBS
  • THBS-1
  • TSP
  • TSP-1
  • TSP1
  • CRISP-2
  • CT36
  • GAPDL5
  • TPX1
  • Thbs-1
  • tbsp1
  • thrombospondin-1
  • thbs
  • thbs-1
  • tsp
  • tsp-1
  • tsp1
  • Tsp1
  • THBS1
  • Tsp-1
  • wu:fc48c03
  • si:dkey-11e23.1
  • 4833423O18Rik
  • Tmtsp
  • thrombospondin, type I, domain containing 1
  • thrombospondin 1
  • cysteine-rich secretory protein 2
  • thrombospondin, type I, domain 1
  • THSD1
  • THBS1
  • CRISP2
  • Thsd1
  • Thbs1
  • thbs1
  • LOC100230636
Alternatives
anti-Mammalian Thrombospondin 1 antibody for Immunofluorescence
Reactivity
Mammalian
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63
59
25
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16
3
3
1
1
1
Host
Mouse
129
43
30
Clonality (Clone)
Monoclonal ()
Conjugate
This Thrombospondin 1 antibody is un-conjugated
28
16
9
4
4
3
1
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
158
103
56
37
33
32
17
17
10
8
4
4
3
3
2
2
2
2
2
2
2
2
Options
Supplier
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Supplier Product No.
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Immunogen BALB/C mice were injected with reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets.
Clone A6-1
Isotype IgG1
Specificity This antibody is specific to 450 kDa protein (non-reduced) and 170 to 180 kDa (reduced) form of Thrombospondin.
Cellular Localization: Secretory granules, Golgi complex, endoplasmic reticulum
Purification Purified
Alternative Name Thrombospondin-1 (THBS1) (THBS1 Antibody Abstract)
Background Thrombospondin is a protein from platelet a granules. It is secreted at sites of platelet activation and aggregation and is involved in the differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.Synonyms: TSP, TSP1
Gene ID 7057
NCBI Accession NP_003237
UniProt P07996
Research Area Extracellular Matrix
Pathways Autophagy
Application Notes Immunofluorescence. Immunoprecipitation. Western Blot. Immunohistochemistry on paraffin embedded sections: diluted of 1: 25-1: 50 in an ABC method (30 minutes at room temperature). Formalin fixedparaffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Recommended Positive Control: Tonsil
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer Sodium azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunohistochemistry (IHC) image for anti-Thrombospondin 1 (THBS1) antibody (ABIN115004) Formalin fixed paraffin embedded human tonsil stained with Thrombospondin antibody. A...
Background publications Galvin, Vance, Dixit, Fink, Frazier: "Interaction of human thrombospondin with types I-V collagen: direct binding and electron microscopy." in: The Journal of cell biology, Vol. 104, Issue 5, pp. 1413-22, 1987 (PubMed).

Dixit, Galvin, ORourke, Frazier: "Monoclonal antibodies that recognize calcium-dependent structures of human thrombospondin. Characterization and mapping of their epitopes." in: The Journal of biological chemistry, Vol. 261, Issue 4, pp. 1962-8, 1986 (PubMed).

Dixit, Haverstick, ORourke, Hennessy, Grant, Santoro, Frazier: "Effects of anti-thrombospondin monoclonal antibodies on the agglutination of erythrocytes and fixed, activated platelets by purified thrombospondin." in: Biochemistry, Vol. 24, Issue 16, pp. 4270-5, 1985 (PubMed).

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