IgE antibody

Antigen: IgE

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN115320

Additional Information

Immunogen: IgE isolated from the serum of a patient with IgE-myeloma.

Cross-Reactivity: Human

Description: IgE is typically the least abundant isotype - blood serum IgE levels in a normal(non-atopic) individual are ~150 ng/ml, compared to 10 mg/ml for the IgGs - it is capableof triggering the most powerful immune reactions. Most of our knowledge of IgE has comefrom research into the mechanism of a form of allergy known as type 1 hypersensitivity. There is much speculation into what physiological benefits IgE contributes, and so far,circumstantial evidence in animal models and statistical population trends have hintedthat IgE may be beneficial in fighting gut parasites such as Schistosoma mansoni, but thishas not been conclusively proven in humans. IgE may play an important role in the immune system's recognition of cancer, in which thestimulation of a strong cytotoxic response against cells displaying only small amounts ofearly cancer markers would be beneficial. IgE may be an important target in treatments forallergy and asthma.

Characteristics: Synonyms: Human Immunoglobulin E

Application Details

Application Notes: Immunohistochemistry on Formalin-Fixed Praffin Embedded Sections: Use at a dilution of1/50-1/500 in an ABC method (30 minutes at room temperature)Recommended Positive Control: Tonsil. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Purified

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Immunology, Secondary Antibodies

Restrictions: For Research Use only

Publications

Skulachev: "Cytochrome c in the apoptotic and antioxidant cascades." in: FEBS letters, Vol. 423, Issue 3, pp. 275-80, 1998 (PubMed).

Hu, Benedict, Wu et al.: "Bcl-XL interacts with Apaf-1 and inhibits Apaf-1-dependent caspase-9 activation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 8, pp. 4386-91, 1998 (PubMed).

Kuida, Haydar, Kuan et al.: "Reduced apoptosis and cytochrome c-mediated caspase activation in mice lacking caspase 9." in: Cell, Vol. 94, Issue 3, pp. 325-37, 1998 (PubMed).