CD309 / VEGFR-2 / Flk-1 antibody

Antigen: CD309 / VEGFR-2 / Flk-1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN115389

Additional Information

Alternative name:

UniProt: P35968

Immunogen: A synthetic peptide derived from the C-terminus of the precursor form of the Human Flk-1

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Description: CD309 is a member of a receptor tyrosine kinase family whose activation plays anessential role in a large number of biological processes such as embryonic development,wound healing, cell proliferation, migration and differentiation. Like other growth factorreceptors, upon ligand binding VEGF receptor 2 dimerises and is autophosphorylated onmultiple tyrosine residues. These sites can be involved in the regulation of kinase activityor serve as binding sites for SH2 and phosphotyrosine binding containing signallingproteins. Phosphorylation of Tyrosines 1054 and 1059 in the activation loop is required foractivation of VEGF receptor 2 and its intrinsic tyrosine kinase activity. In case of HIV-1infection, the interaction with extracellular viral Tat protein seems to enhanceangiogenesis in Kaposi's sarcoma lesions.

Characteristics: Synonyms: VEGFR2, FLK1, KDR, VEGF Receptor 2, Vascular endothelial growth factor receptor 2,Protein-tyrosine kinase receptor Flk-1, Kinase insert domain receptor, Kinase NYK

Application Details

Application Notes: Western Blotting. Immunohistochemistry on Paraffin Sections: Use a 1/50-1/100 dilution in an ABC method(incubation time: 30 minutes at room temperature). Formalin fixed paraffin embedded tissue sections require high temperature antigenunmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Recommended positive control: Angiosarcoma. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Purified

Storage: Store the antibody undiluted at 2-8°C. Shelf life: one year from despatch.

Research Area: Cytokines, Receptors

Restrictions: For Research Use only

Images

Formalin fixed paraffin embedded human angiosarcoma stained with Flk-1 antibody

Publications

Quinn, Peters, De Vries et al.: "Fetal liver kinase 1 is a receptor for vascular endothelial growth factor and is selectively expressed in vascular endothelium." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 16, pp. 7533-7, 1993 (PubMed).

Erguen, Kilic, Fiedler et al.: "Vascular endothelial growth factor and its receptors in normal human testicular tissue." in: Molecular and cellular endocrinology, Vol. 131, Issue 1, pp. 9-20, 1997 (PubMed).