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Leptin antibody (LEP)

Details for Product anti-LEP Antibody No. ABIN115507, Supplier: Login to see
Antigen
  • ob
  • obese
  • LEPD
  • OB
  • OBS
Alternatives
anti-Rat (Rattus) Leptin antibody for ELISA
Reactivity
Rat (Rattus)
345
155
133
18
3
1
1
1
Host
Rabbit
357
141
20
10
4
Clonality
Polyclonal
Conjugate
This Leptin antibody is un-conjugated
60
19
13
7
7
5
5
3
3
3
3
3
3
3
3
2
2
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
349
333
69
40
39
33
26
25
18
17
16
16
10
7
5
3
3
3
2
2
1
1
1
1
1
1
Supplier
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Immunogen Recombinant rat Leptin (E. coli).
Specificity This antibody reacts to Leptin.
Purification Immunoaffinity chromatography
Alternative Name Leptin (LEP Antibody Abstract)
Background Leptin, the product of the ob (obese) gene, is a single-chain 16 kDa proteohormone consisting of 146 amino acid residues. Leptin is produced by differentiated adiocytes, although production have been demonstrated in other tissues, such as fundus of the stomach, the sceletal muscle, the liver, and the placenta. Leptin is considered to play an important role in appetite control, fat metabolism and body weight regulation. It targets the central nervous system, in particular the hypothalamus, suppressing food intake and stimulating energy expenditure. In humans, leptin levels correlate with body mass index (BMI) and percentage body fat, and are elevated even in obese individuals. Leptin has a dual action, it decreases the appetite and increases energy consumption, causing more fat to be burned.Synonyms: LEP, OB, OBS, Obese protein, Obesity factor
Gene ID 25608
NCBI Accession NP_037208
UniProt P50596
Research Area Cardiovascular, Atherosclerosis, Hormones
Pathways JAK-STAT Signaling, AMPK Signaling
Application Notes ELISA. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore with 0.1 mL of deionized water
Concentration 1.0 mg/mL
Buffer 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE
Preservative Azide free
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 months.
Expiry Date 6 months
Background publications Cong, Chen, Li et al.: "Regulation of adiponectin and leptin secretion and expression by insulin through a PI3K-PDE3B dependent mechanism in rat primary adipocytes." in: The Biochemical journal, Vol. 403, Issue 3, pp. 519-25, 2007 (PubMed).

Ricci, Lee, Russell et al.: "Isoproterenol decreases leptin release from rat and human adipose tissue through posttranscriptional mechanisms." in: American journal of physiology. Endocrinology and metabolism, Vol. 288, Issue 4, pp. E798-804, 2005 (PubMed).

Maggini, Spila Alegiani, Di Giovambattista et al.: "[Patterns of use of hormone replacement therapy in 2 Italian populations]" in: Annali dell'Istituto superiore di sanità, Vol. 33, Issue 2, pp. 195-7, 1998 (PubMed).

Considine, Sinha, Heiman et al.: "Serum immunoreactive-leptin concentrations in normal-weight and obese humans." in: The New England journal of medicine, Vol. 334, Issue 5, pp. 292-5, 1996 (PubMed).

Halaas, Gajiwala, Maffei et al.: "Weight-reducing effects of the plasma protein encoded by the obese gene." in: Science (New York, N.Y.), Vol. 269, Issue 5223, pp. 543-6, 1995 (PubMed).

Lönnqvist, Arner, Nordfors et al.: "Overexpression of the obese (ob) gene in adipose tissue of human obese subjects." in: Nature medicine, Vol. 1, Issue 9, pp. 950-3, 1995 (PubMed).

Maffei, Halaas, Ravussin et al.: "Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects." in: Nature medicine, Vol. 1, Issue 11, pp. 1155-61, 1995 (PubMed).