IL8
Reactivity: Human
WB, ELISA, IHC (p), IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: In a Sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL ofthis antibody will detect at least 1000 pg/mL of recombinant Human IL-8 when used withbiotinylated antigen affinity purified anti-Human IL-8 (cat. no. PP1030B) as the detectionantibody at a concentration of approximately 0.5-1.0 μg/mL. Western Blot: To detect Human IL-8, this antibody can be used at a concentration of0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hIL-8 is 2.0-4.0 ng/lane, under non-reducing conditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore in sterile water to a concentration of 1.0 mg/mL.
Buffer
PBS without preservatives
Preservative
Without preservative
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
The lyophilized antibody is stable at room temperature for one month and at -20 °C for one year from despatch. The reconstituted antibody is stable for two weeks at 2-8 °C. Frozen aliquots are stable for six months when stored at -20 °C.
IL8 is a member of the CXC chemokine family. This family of small basic heparan-binding proteins are proinflammatory and primarily mediate the activation and migration of neutrophils into tissue from peripheral blood. This chemokine is one of the major mediators of the inflammatory response and is secreted by several cell types in response to an inflammatory stimulus. It functions as a chemoattractant, and is also a potent angiogenic factor. IL8 attracts neutrophils, basophils, and T-cells, but not monocytes. Cystic fibrosis (CF) is characterized by severe lung inflammation. The inflammatory process is believed to be caused by massive overproduction of the proinflammatory protein IL8, and the high levels of IL8 in the CF lung are therefore believed to be the central mechanism behind CF lung pathophysiology.Synonyms: CXCL8, Emoctakin, GCP1, Granulocyte chemotactic protein 1, MDNCF, MONAP, Monocyte-derived neutrophil chemotactic factor, Monocyte-derived neutrophil-activating peptide, NAP1, Neutrophil-activating protein 1, Protein 3-10C, T-cell chemotactic factor