CCL2 antibody

Catalog No. ABIN115767

Product Details anti-CCL2 Antibody

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CCL2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purification: Affinity Chromatography on Protein A

Immunogen: Highly pure (> 98%) E.Coli derived Recombinant Human MCP-1/MCAF (Cat.-No PA098)

Isotype: IgG1

Application Details

Application Notes: ELISA: In a Sandwich ELISA (assuming 100 μL/well), a concentration of 2-4 μg/mL of thisantibody will detect at least 300 pg/mL of recombinant Human MCP1 when used withBiotinynaled affinity purified anti-Human MCP1 antibody (PP1044B1 or PP1044B2). Western Blot: To detect Human MCP1 by Western Blot analysis this antibody can be used ata concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant Human MCP1 is 1.0-2.0 ng/lane, underreducing or non-reducing conditions. Immunohistochemistry: 10 μg/mL with an Overnight Incubation at 4 °C. This MCP1 antibody stained Formalin-Fixed, Paraffin-Embedded Sections of Human breastinvasive ductal carcinoma. An HRP-labeled polymer detection system was used with a DABChromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer isrecommended.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with sterile water to a concentration of 1.0 mg/mL.

Buffer: PBS without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CCL2 antibody (ABIN115767)

Betz, Blood, Blood: "University students' perceptions of pre-school and kindergarten children who stutter." in: Journal of communication disorders, Vol. 41, Issue 3, pp. 259-73, (2008) (PubMed).

Target Details for CCL2

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Alternative Name: MCP1 / CCL2 (CCL2 Products)

Synonyms: GDCF-2 antibody, HC11 antibody, HSMCR30 antibody, MCAF antibody, MCP-1 antibody, MCP1 antibody, SCYA2 antibody, SMC-CF antibody, AI323594 antibody, JE antibody, Scya2 antibody, Sigje antibody, MCP-1A antibody, MCP1A antibody, C-C motif chemokine ligand 2 antibody, chemokine (C-C motif) ligand 2 antibody, C-C motif chemokine 2 antibody, CCL2 antibody, Ccl2 antibody, LOC101120093 antibody

Background: This chemotactic factor attracts monocytes and basophils but not neutrophils or eosinophils and augments monocyte anti-tumor activity. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. It may be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.Synonyms: C-C motif chemokine 2, HC-11, HC11, MCAF, MCP-1, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, SCYA2, Small-inducible cytokine A2

Gene ID: 9606

UniProt: P13500

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, The Global Phosphorylation Landscape of SARS-CoV-2 Infection