CCL21 antibody

Catalog No. ABIN116001

Product Details anti-CCL21 Antibody

Target: CCL21 (Chemokine (C-C Motif) Ligand 21 (CCL21))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL21 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Specificity: This antibody recognizes Human Exodus-2.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography.

Immunogen: Highly pure (>98%) recombinant Human Exodus-2.

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofHuman Exodus-2 (100.0 ng/mL), a concentration of 6.0-8.0 μg/mL of this antibody isrequired. ELISAIndirect: To detect Human Exodus-2 by indirect ELISA (using 100 μL/well antibody solution)a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant Human Exodus-2. Sandwich: To detect Human Exodus-2 by sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with Biotinylated Anti-Human Exodus-2 (PP1005B1 orPP1005B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well ofrecombinant Human Exodus-2. Western Blot: To detect Human Exodus-2 by Western Blot analysis this antibody can beused at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant Human Exodus-2 is 1.5-3.0 ng/lane, under

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening! This product is photosensitive and should be protected from light

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2-8 °C for one month or at -20 °C for longer.

Target Details for CCL21

Target: CCL21 (Chemokine (C-C Motif) Ligand 21 (CCL21))

Alternative Name: 6CKine / CCL21 (CCL21 Products)

Synonyms: 6Ckine antibody, CKb9 antibody, ECL antibody, SCYA21 antibody, SLC antibody, TCA4 antibody, ccl21 antibody, Ccl21b antibody, C-C motif chemokine ligand 21 antibody, C-C motif chemokine 21 antibody, CCL21 antibody, ccl21 antibody, Ccl21 antibody

Background: Exodus 2 is one of several CC cytokine genes clustered on the p arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterised by two adjacent cysteines. Similar to other chemokines the protein encoded by this gene inhibits hemopoiesis and stimulates chemotaxis. This protein is chemotactic in vitro for thymocytes and activated T cells, but not for B cells, macrophages, or neutrophils. The cytokine encoded by this gene may also play a role in mediating homing of lymphocytes to secondary lymphoid organs. It is a high affinity functional ligand for chemokine receptor 7 (CCR7) that is expressed on T and B lymphocytes and a known receptor for another member of the cytokine family (small inducible cytokine A19).Synonyms: Beta chemokine Exodus-2, CCL-21, SCYA21, SLC, Secondary lymphoid-tissue chemokine, Small-inducible cytokine A21

Gene ID: 6366

NCBI Accession: NP_002980

UniProt: O00585

Pathways: Regulation of Actin Filament Polymerization