Interferon gamma antibody

Catalog No. ABIN116053

Product Details anti-Interferon gamma Antibody

Target: Interferon gamma (IFNG)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Interferon gamma antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func), Enzyme Immunoassay (EIA)

Purification: Affinity Chromatography using immobilized Human IFN-gamma matrix

Immunogen: Highly purified (>98%) E.coli derived recombinant Human Inferferon gamma (Cat.-No PA070)

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofh-IFN-gamma (50 pg/mL), a concentration of 0.04-0.06 μg/mL of the previous lot of thisantibody was required. ELISA: To detect h-IFN-gamma by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant h-IFN-gamma. Western blot: To detect h-IFN-gamma by Western Blot analysis this antibody can be used ata concentration of 0.1- 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant h-IFN-gamma is 1.5-3.0 ng/lane, under either reducingor non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for Interferon gamma

Target: Interferon gamma (IFNG)

Alternative Name: IFNG / Interferon gamma (IFNG Products)

Synonyms: IFG antibody, IFI antibody, IFN-g antibody, Ifg antibody, IFNG2 antibody, IFN-gamma antibody, IFN-G antibody, IFNG antibody, IFNgamma antibody, TCRalpha antibody, INF-G antibody, ifng antibody, interferon gamma antibody, interferon, gamma 1-2 antibody, IFNG antibody, Ifng antibody, ifng1-2 antibody

Background: Interferon gamma is mainly produced by T lymphocytes and NK cells. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses,including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak antiviral and antiproliferative activity, and poteniates the antiviral and anti tumor effects of IFN alpha / beta (type I interferon). It is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. Labile at pH 2.Synonyms: IFN-gamma, gamma IFN

Gene ID: 9606

UniProt: P01579

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy