CCL22 antibody

Catalog No. ABIN116160

Product Details anti-CCL22 Antibody

Target: CCL22 (Chemokine (C-C Motif) Ligand 22 (CCL22))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL22 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: Human Macrophage Derived Chemokine (MDC, CCL22)

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hMDC (human Macrophage-Derived Chemokine).

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhMDC (100 ng/mL), a concentration of 5-7 μg/mL of this antibody is required. ELISA: To detect hMDC by direct ELISA (using 100l/well antibody solution) a concentrationof at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, inconjunction with compatible secondary reagents, allows the detection of of 0.2-0.4 ng/well of recombinant hMDC. Western Blot: To detect hMDC by Western Blot analysis this antibody can be used at aconcentration of 0.1- 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hMDC is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Prior to reconstitution best stored desiccated.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior and following at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for CCL22

Target: CCL22 (Chemokine (C-C Motif) Ligand 22 (CCL22))

Alternative Name: MDC / CCL22 (CCL22 Products)

Synonyms: ABCD-1 antibody, DC/B-CK antibody, MDC antibody, SCYA22 antibody, STCP-1 antibody, DCBCK antibody, Scya22 antibody, Mdc antibody, CCL22 antibody, C-C motif chemokine ligand 22 antibody, chemokine (C-C motif) ligand 22 antibody, CCL22 antibody, Ccl22 antibody

Background: The MDC gene is one of several Cys-Cys (CC) cytokine genes which are clustered on the q arm of chromosome 16. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by MDC displays chemotactic activity for natural killer cells, chronically activated T lymphocytes, monocytes and dendritic cells. It has no chemoattractant activity for eosinophils, neutrophils and resting T lymphocytes and also displays a mild activity for primary activated T lymphocytes. The product of this gene binds to chemokine receptor CCR4. This chemokine may play a role in the trafficking of activated T lymphocytes to inflammatory sites and other aspects of activated T lymphocyte physiology.Synonyms: C-C motif chemokine 22, CC chemokine STCP-1, Macrophage-derived chemokine, SCYA22, Small-inducible cytokine A22, Stimulated T-cell chemotactic protein 1

Gene ID: 6367

NCBI Accession: NP_002981

UniProt: O00626