Thrombopoietin antibody
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- Target See all Thrombopoietin (THPO) Antibodies
- Thrombopoietin (THPO)
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This Thrombopoietin antibody is un-conjugated
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)
- Specificity
- This antibody reacts with Thrombopoietin.
- Purification
- Affinity chromatography.
- Immunogen
- Highly purified recombinant human TPO.
- Top Product
- Discover our top product THPO Primary Antibody
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- Application Notes
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Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhTPO (5.00 ng/mL), a concentration of 4.2-6.3 μg/mL of this antibody is required. ELISA: (Indirect): To detect hTPO by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2-0.4 ng/well of recombinant hTPO. (Sandwich): To detect hTPO by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Human TPO (PP1074B) as a detectionantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTPO. Western Blot: To detect hTPO by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hTPO is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Reconstitution
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
- Buffer
- PBS, pH 7.2 without preservatives.
- Preservative
- Without preservative
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- Target
- Thrombopoietin (THPO)
- Alternative Name
- Thrombopoietin (THPO Products)
- Synonyms
- MGDF antibody, MKCSF antibody, ML antibody, MPLLG antibody, THCYT1 antibody, TPO antibody, Mgdf antibody, Ml antibody, Mpllg antibody, Tpo antibody, Tpo1 antibody, Tpo2 antibody, Tpo3 antibody, Tpo4 antibody, tpo antibody, LOC100231762 antibody, thrombopoietin antibody, THPO antibody, Thpo antibody, thpo antibody
- Background
- Synonyms: C-mpl ligand, MGDF, Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, Myeloproliferative leukemia virus oncogene ligand, THPO
- Gene ID
- 7066
- NCBI Accession
- NP_000451
- UniProt
- P40225
- Pathways
- JAK-STAT Signaling, Hormone Activity
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