CCL16
Reactivity: Human
ELISA
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: To detect hLEC by direct ELISA (using 100 l/well antibody solution) this antibodycan be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant hLEC. Western Blot: To detect hLEC by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hLEC is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
Buffer
PBS, pH 7.2 without preservatives.
Preservative
Without preservative
Handling Advice
Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
Storage
4 °C/-20 °C
Storage Comment
Store the antibody diluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
LEC (liver expressed cytokine) displays chemotactic activity for lymphocytes and monocytes but not for neutrophils. It also shows a potent myelosuppressive activity and suppresses proliferation of myeloid progenitor cells. Its expression is upregulated by IL10.Synonyms: C-C motif chemokine 16, Chemokine CC-4, Chemokine LEC, IL-10-inducible chemokine, ILINCK, LCC-1, LCC1, Liver-expressed chemokine, Lymphocyte and monocyte chemoattractant, Monotactin-1, NCC-4, SCYA16