Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, beta (NFKBIB) (C-Term) antibody

Details for Product No. ABIN116525
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Antigen
Synonyms NFKBIB, IKB-beta, IKappaBbeta, IkB, IkBb, IKBB, TRIP9
Epitope
C-Term
(29), (16), (12), (12), (7), (5), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(135), (68), (67), (36), (28), (28)
Host
Rabbit
(112), (24)
Clonality
Polyclonal
Conjugate
Un-conjugated
(6), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2)
Application
ELISA, Immunoprecipitation (IP), Western Blotting (WB)
(76), (40), (40), (38), (26), (8), (8), (6), (3), (2), (1)
Pubmed 1 reference available
Quantity 0.1 mL
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Catalog No. ABIN116525
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Immunogen IkB beta peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Specificity Recognition is at the highly conserved C-terminal region. Anti-IkappaBbeta may react non- specifically with other proteins. Control peptide (R1014CP) will compete only with the specific reaction of antiserum with IkappaBbeta.
Purification Prepared from monospecific antiserum by delipidation and defibrination
Alternative Name NFKBIB / IKBB
Background NFkappaB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFkappaB bound to IkappaB. NFkappaB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits. Other identified subunits include p52 (NFKB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFkappaB subunit p65, similar to p50/p65 heterodimers. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IkappaB-alpha. IkappaB-alpha binds to the p65 subunit, preventing nuclear localization and DNA binding. Low levels of p52 and p50 homodimers can also exist in cells.
Alternate names: I-kappa-B-beta, IkB-B, IkB-beta, IkappaBbeta, NF-kappa-B inhibitor beta, TR-interacting protein 9, TRIP9, Thyroid receptor-interacting protein 9
Gene ID 4793
NCBI Accession NP_001001716
UniProt Q15653
Application Notes Suitable for Immunoprecipitation, Western blotting (171,000, shows a 45kD band), ELISAand Dot Blot. Recommended Dilutions: This product was assayed by immunoblot and found to bereactive against IκBβ at a dilution of 1: 1000 followed by reaction with Peroxidaseconjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat). Anti-IκBβ is suitable for thedetection by immunoblot of human, mouse and rat IκBβ. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 90 mg/mL (by Refractometry)
Handling Advice Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage 4 °C/-20 °C
Storage Comment Store lyophilized product at 2-8°C. After reconstitution, store at -20°C.
Expiry Date 12 months
Background publications Baldwin: "The NF-kappa B and I kappa B proteins: new discoveries and insights." in: Annual review of immunology, Vol. 14, Issue 8, pp. 649-83, 1996 (PubMed).

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