NFYB antibody (N-Term)

Catalog No. ABIN116537

Product Details anti-NFYB Antibody

Target: NFYB (Nuclear Transcription Factor Y, beta (NFYB))

Binding Specificity: N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFYB antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Electrophoretic Mobility-Shift Assay (EMSA), Enzyme Immunoassay (EIA)

Purification: This product was prepared from monospecific antiserum by delipidation and defibrination.

Immunogen: NF-Y (B subunit specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Application Details

Application Notes: Suitable for Immunoprecipitation, Immunoblotting, ELISA and supershift assays. Recommended Dilutions: This product was assayed by immunoblot and found to bereactive against NF-Y (B subunit specific) at a dilution of 1: 2,000 followed by reaction withPeroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat). Anti-NF-Y (B subunit specific) is suitable for the detection by Immunoblot of human andmouse NF-Y. Minimal reactivity was observed against the A subunit of NF-Y. This product was also tested in a gel supershift assay and found to be reactive against NF-Yusing 0.5 to 1.0 mLper assay.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 90.0 mg/mL (by Refractometry)

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store original vial at 2-8 °C. After reconstitution store at 2-8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for NFYB

Target: NFYB (Nuclear Transcription Factor Y, beta (NFYB))

Alternative Name: NFYB (NFYB Products)

Synonyms: CBF-A antibody, CBF-B antibody, HAP3 antibody, NF-YB antibody, NFYB antibody, nfyb antibody, zgc:110533 antibody, zgc:110552 antibody, hap3 antibody, cbf-a antibody, cbf-b antibody, CBFB antibody, Nfya antibody, AA985999 antibody, Cbf-A antibody, nuclear transcription factor Y subunit beta antibody, nuclear transcription factor Y subunit beta L homeolog antibody, nuclear transcription factor Y, beta b antibody, nuclear transcription factor Y, beta a antibody, nuclear transcription factor-Y beta antibody, NFYB antibody, nfyb.L antibody, nfybb antibody, nfyba antibody, nfyb antibody, Nfyb antibody

Background: The Y box is a CCAAT box which is bound by the heteromeric DNA binding protein, NFY (also known as CBF and CP1). Unlike the transcription factors C/EBP and CTF/NF1 which also bind CCAAT-like sequences, NFY exhibits a strict binding requirement for this pentanucleotide sequence. Binding sites for this factor have been described for nearly 30 % of all eukaryotic genes. Y/CCAAT sequences were frequently observed in the promoter-proximal sequences. NF-Y is composed of 3 separate subunits (A,B and C) each of which is required for DNA binding. Each subunit has remained highly conserved throughout evolution. In fact, homologous yeast subunits can substitute for mammalian NF-Y in DNA-binding assays. The conserved core sequences of NF-YB and NF-YC contain a 70 aa region similar to the histone fold motif of nucleosomes H2A and H2B. The unique structure and evolutionary conservation of this transcription factor suggests that it plays a fundamental role in the readout of eukaryotic genetic information.Synonyms: HAP3, Nuclear transcription factor Y subunit beta

Gene ID: 4801

NCBI Accession: NP_006157

UniProt: P25208

Pathways: Regulation of Lipid Metabolism by PPARalpha