TAP1 antibody (C-Term)

Catalog No. ABIN116539

This Rabbit Polyclonal antibody specifically detects TAP1 in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for TAP1 antibody (C-Term) (ABIN116539)

Target: TAP1 (Transporter 1, ATP-Binding Cassette, Sub-Family B (MDR/TAP) (TAP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TAP1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-TAP1 Antibody

Binding Specificity: C-Term

Specificity: A single precipitin arc was observed against anti-Rabbit Serum when assayed by immunoelectrophoresis.

Purification: Prepared from monospecific antiserum by delipidation, salt fractionation and ion exchange chromatography.

Immunogen: Transporter Associated Protein (TAP I) peptide corresponding to the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Application Details

Application Notes: Suitable for Immunoblotting, Immunoprecipitation, Immunofluorescence, Flow cytometryand ELISA. Recommended Dilutions: This product was assayed by ELISA against TAP-I peptide in anantibody sandwich assay using Peroxidase conjugated Affinity Purified anti-Rabbit IgG[H&L] (Goat). A dilution of 1: 10,000 is suggested from this experiment. No reaction wasobserved against TAP-II. This product was assayed by immunoblotting and was reactive against the 72 kDa humanTAP-I protein at a dilution of 1: 1,000 followed by reaction with Peroxidase conjugatedAffinity Purified anti-Rabbit IgG [H&L] (Goat). No reaction was observed against TAP-II.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % sodium azide as a preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store lyophilized product at 2-8 °C. After reconstitution, store at -20 °C.

Target Details for TAP1

Target: TAP1 (Transporter 1, ATP-Binding Cassette, Sub-Family B (MDR/TAP) (TAP1))

Alternative Name: ABCB2 / APT1 / TAP1

Background: Synonyms: ATP-binding cassette sub-family B member 2, Antigen peptide transporter 1, PSF1, Peptide supply factor 1, Peptide transporter PSF1, Peptide transporter involved in antigen processing 1, RING4, Y3

Gene ID: 6890

NCBI Accession: NP_000584

UniProt: Q03518

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Human Leukocyte Antigen (HLA) in Adaptive Immune Response