Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Carboxypeptidase A [Bovine Pancreas]. Cross reactivity against Carboxypeptidase A from other tissues and species may occur but have not been specifically determined.
Purification
Prepared from monospecific antiserum by a delipidation and defibrination.
CPA1
Reactivity: Human
WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Suitable for Immunoblotting (Western blot: 1/500-1/3,000or Dot blot), ELISA (1/5,000-1/25,000), Immunoprecipitation and most immunologicalmethods requiring high titer and specificity. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Reconstitution
Restore with 2.0 mL of deionized water (or equivalent).
Concentration
90.0 mg/mL (by Refractometry)
Buffer
0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.6, without preservative.
Preservative
Without preservative
Handling Advice
Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
Storage
4 °C/-20 °C
Storage Comment
Store vial at 2-8 ° C prior to restoration. Following restoration product can be stored undiluted at 2-8 ° for up to one month or (in aliquots) at -20 °C or below.
Carboxypeptidase A is a pancreatic exopeptidase. Three different forms of human pancreatic procarboxypeptidase A have been isolated. The A1 and A2 forms are monomeric proteins with different biochemical properties.Synonyms: CPA, CPA1