Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Lactoperoxidase [Bovine Milk]. Cross reactivity against Lactoperoxidase from other tissues and species may occur but have not been specifically determined.
Purification
Prepared from monospecific antiserum by a delipidation and defibrination.
LPO
Reactivity: Human
ELISA
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Suitable for Immunoblotting (Western Blot: 1/2,000-1/10,000, or Dot blot), ELISA(1/18,000-1/75,000), Immunoprecipitation and most immunological methods requiringhigh titer and specificity. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Reconstitution
Restore with 2.0 mL of deionized water (or equivalent).
Concentration
90.0 mg/mL (by Refractometry)
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing. Dilute only prior to immediate use
Storage
4 °C/-20 °C
Storage Comment
Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.