Tubulin-GTP antibody

Details for Product No. ABIN1169388, Supplier: Log in to see
Antigen
Reactivity
Fruit Fly (Drosophila melanogaster), Human, Mouse (Murine), Rat (Rattus)
2
1
1
1
1
Host
Human
1
1
Antibody Type
Recombinant Antibody
Clonality (Clone)
Monoclonal ()
Application
Immunocytochemistry (ICC)
1
1
Options
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Immunogen Full length GTP-lambda-S loaded tubulin from pig brain.
Clone MB11
Isotype IgG2
Fragment lambda
Specificity Recognizes human, mouse, rat and drosophila tubulin-GTP.
Cross-Reactivity Fruit Fly (Drosophila melanogaster), Human, Mouse (Murine), Rat (Rattus)
Purification Purified from HEK 293 cell culture supernatant.
Purity >95 % (SDS-PAGE)
Background Anti-Tubulin-GTP, monoclonal antibody (recombinant) (MB11) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.
Application Notes Optimal working dilution should be determined by the investigator.
Comment

anti-Tubulin-GTP, monoclonal antibody (recombinant) (MB11) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain.

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer In PBS containing 10 % glycerol and 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.
Expiry Date 12 months
Product cited in: Nakata, Niwa, Okada, Perez, Hirokawa: "Preferential binding of a kinesin-1 motor to GTP-tubulin-rich microtubules underlies polarized vesicle transport." in: The Journal of cell biology, Vol. 194, Issue 2, pp. 245-55, 2011 (PubMed).

Dimitrov, Quesnoit, Moutel, Cantaloube, Poüs, Perez: "Detection of GTP-tubulin conformation in vivo reveals a role for GTP remnants in microtubule rescues." in: Science (New York, N.Y.), Vol. 322, Issue 5906, pp. 1353-6, 2008 (PubMed).

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