BMP4 antibody (AA 293-408) (Biotin)
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- Target See all BMP4 Antibodies
- BMP4 (Bone Morphogenetic Protein 4 (BMP4))
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Binding Specificity
- AA 293-408
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This BMP4 antibody is conjugated to Biotin
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA
- Sequence
- MGHHHHHHSG S-SPKHHSQR ARKKNKNCRR HSLYVDFSDV GWNDWIVAPP GYQAFYCHGD CPFPLADHLN STNHAIVQTL VNSVNSSIPK ACCVPTELSA ISMLYLDEYD KVVLKNYQEM VVEGCGCR
- Specificity
- It has been selected for its ability to recognize BMP4 in immunohistochemical staining and Western blotting.
- Purification
- Affinity Chromatography
- Immunogen
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Recombinant BMP4 expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against BMP4 conjugated to biotin. - Isotype
- IgG
- Top Product
- Discover our top product BMP4 Primary Antibody
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- Application Notes
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Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user. - Comment
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Content: The quality control contains recombinant BMP4 (Ser293~Arg408) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % . - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze/thaw cycles
- Storage
- 4 °C
- Storage Comment
- Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
- Expiry Date
- 12 months
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- Target
- BMP4 (Bone Morphogenetic Protein 4 (BMP4))
- Abstract
- BMP4 Products
- Synonyms
- BMP2B antibody, BMP2B1 antibody, MCOPS6 antibody, OFC11 antibody, ZYME antibody, Bmp-4 antibody, Bmp2b antibody, Bmp2b-1 antibody, Bmp2b1 antibody, BOMPR4A antibody, bmp-4 antibody, zbmp-4 antibody, zgc:100779 antibody, BMP-4 antibody, XBMP-4 antibody, bmp2b antibody, bmp2b1 antibody, bmp4 antibody, ofc11 antibody, xbmp4 antibody, zyme antibody, BMP4 antibody, bone morphogenetic protein 4 antibody, bone morphogenetic protein 4 L homeolog antibody, bone morphogenetic protein 4 S homeolog antibody, BMP4 antibody, Bmp4 antibody, bmp4 antibody, bmp4.L antibody, bmp4.S antibody
- Pathways
- Steroid Hormone Mediated Signaling Pathway, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
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