KRT16 antibody (AA 117-419) (Biotin)
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- Target See all KRT16 Antibodies
- KRT16 (Keratin 16 (KRT16))
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Binding Specificity
- AA 117-419
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This KRT16 antibody is conjugated to Biotin
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA
- Sequence
- MHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTDD DDKAMADIGSEF-EKVT MQNLNDRLAS YLDKVRALEE ANADLEVKIR DWYQRQRPSE IKDYSPYFKT IEDLRNKIIA ATIENAQPIL QIDNARLAAD DFRTKYEHEL ALRQTVEADV NGLRRVLDEL TLARTDLEMQ IEGLKEELAY LRKNHEEEML ALRGQTGGDV NVEMDAAPGV DLSRILNEMR DQYEQMAEKN RRDAETWFLS KTEELNKEVA SNSELVQSSR SEVTELRRVL QGLEIELQSQ LSMKASLENS LEETKGRYCM QLSQIQGLIG SVEEQLAQLR CEMEQQSQEY QILLDVKTRL EQEIATYRR
- Specificity
- It has been selected for its ability to recognize KRT16 in immunohistochemical staining and Western blotting.
- Purification
- Affinity Chromatography
- Immunogen
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Recombinant KRT16 expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against KRT16 conjugated to biotin. - Isotype
- IgG
- Top Product
- Discover our top product KRT16 Primary Antibody
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- Application Notes
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Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user. - Comment
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Content: The quality control contains recombinant KRT16 (Glu117~Arg419) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % . - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze/thaw cycles
- Storage
- 4 °C
- Storage Comment
- Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
- Expiry Date
- 12 months
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- Target
- KRT16 (Keratin 16 (KRT16))
- Abstract
- KRT16 Products
- Synonyms
- CK16 antibody, FNEPPK antibody, K16 antibody, K1CP antibody, KRT16A antibody, NEPPK antibody, AI324768 antibody, Krt1-16 antibody, Ka16 antibody, KRT16 antibody, Krt16 antibody, keratin 16 antibody, keratin 16, type I S homeolog antibody, keratin, type I cytoskeletal 16 antibody, KRT16 antibody, Krt16 antibody, krt16.S antibody, LOC101108147 antibody, LOC100714013 antibody
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