CA5A antibody (AA 12-219)

Catalog No. ABIN1175977

Product Details anti-CA5A Antibody

Target: CA5A (Carbonic Anhydrase VA (CA5A))

Binding Specificity: AA 12-219

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CA5A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Carbonic Anhydrase VA (CA5A)

Specificity: The antibody is a rabbit polyclonal antibody raised against CA5A. It has been selected for its ability to recognize CA5A in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Carbonic Anhydrase VA (CA5A) corresdonding to Tyr12~Leu219 (Accession # P43165)

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.2-2 μg/mL

1:250-2500 Immunohistochemistry: 5-20 μg/mL

1:25-100 Immunocytochemistry: 5-20 μg/mL

1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for CA5A

Target: CA5A (Carbonic Anhydrase VA (CA5A))

Alternative Name: Carbonic Anhydrase VA (CA5A Products)

Synonyms: CAV antibody, Ca5a antibody, Car5 antibody, Ca5 antibody, CA5 antibody, CAVA antibody, carbonic anhydrase 5a, mitochondrial antibody, carbonic anhydrase 5A antibody, Car5a antibody, Ca5a antibody, CA5A antibody

Background: CA-VA, CAVA, CAV, CA5, Carbonate dehydratase 5A, mitochondrial