CD31 antibody

Catalog No. ABIN1176844

Product Details anti-CD31 Antibody

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD31 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)), Blocking Reagent (BR)

Brand: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: 129/Sv mouse-derived endothelioma cell line tEnd.1

Clone: MEC 13-3

Isotype: IgG2a kappa

Application Details

Application Notes: For IHC, we recommend the use of purified MEC13.3 mAb in our special formulation for immunohistochemistry, Cat. No. 550274.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-CD31 antibody (ABIN1176844)

Horenstein, Stockinger, Imhof, Malavasi: "CD38 binding to human myeloid cells is mediated by mouse and human CD31." in: The Biochemical journal, Vol. 330 ( Pt 3), pp. 1129-35, (1998) (PubMed).

DeLisser, Christofidou-Solomidou, Strieter, Burdick, Robinson, Wexler, Kerr, Garlanda, Merwin, Madri, Albelda: "Involvement of endothelial PECAM-1/CD31 in angiogenesis." in: The American journal of pathology, Vol. 151, Issue 3, pp. 671-7, (1997) (PubMed).

Ling, Luxenberg, Wang, Nickbarg, Leenen, Neben, Kobayashi: "Structural identification of the hematopoietic progenitor antigen ER-MP12 as the vascular endothelial adhesion molecule PECAM-1 (CD31)." in: European journal of immunology, Vol. 27, Issue 2, pp. 509-14, (1997) (PubMed).

Suri, Jones, Patan, Bartunkova, Maisonpierre, Davis, Sato, Yancopoulos: "Requisite role of angiopoietin-1, a ligand for the TIE2 receptor, during embryonic angiogenesis." in: Cell, Vol. 87, Issue 7, pp. 1171-80, (1997) (PubMed).

Vanzulli, Gazzaniga, Braidot, Vecchi, Mantovani, Wainstok de Calmanovici: "Detection of endothelial cells by MEC 13.3 monoclonal antibody in mice mammary tumors." in: Biocell : official journal of the Sociedades Latinoamericanas de Microscopi?a Electronica ... et. al, Vol. 21, Issue 1, pp. 39-46, (1997) (PubMed).

Piali, Hammel, Uherek, Bachmann, Gisler, Dunon, Imhof: "CD31/PECAM-1 is a ligand for alpha v beta 3 integrin involved in adhesion of leukocytes to endothelium." in: The Journal of cell biology, Vol. 130, Issue 2, pp. 451-60, (1995) (PubMed).

DeLisser, Newman, Albelda: "Molecular and functional aspects of PECAM-1/CD31." in: Immunology today, Vol. 15, Issue 10, pp. 490-5, (1994) (PubMed).

Rosenblum, Murata, Nelson, Werner, Ranken, Harmon: "Anti-CD31 delays platelet adhesion/aggregation at sites of endothelial injury in mouse cerebral arterioles." in: The American journal of pathology, Vol. 145, Issue 1, pp. 33-6, (1994) (PubMed).

Vecchi, Garlanda, Lampugnani, Resnati, Matteucci, Stoppacciaro, Schnurch, Risau, Ruco, Mantovani: "Monoclonal antibodies specific for endothelial cells of mouse blood vessels. Their application in the identification of adult and embryonic endothelium." in: European journal of cell biology, Vol. 63, Issue 2, pp. 247-54, (1994) (PubMed).

Target Details for CD31

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Alternative Name: CD31 (PECAM-1) (PECAM1 Products)

Synonyms: CD31 antibody, CD31/EndoCAM antibody, GPIIA' antibody, PECA1 antibody, PECAM-1 antibody, endoCAM antibody, C85791 antibody, Cd31 antibody, Pecam antibody, platelet/endothelial cell adhesion molecule 1 antibody, platelet and endothelial cell adhesion molecule 1 antibody, pecam1 antibody, PECAM1 antibody, Pecam1 antibody

Background: The MEC13.3 antibody reacts with CD31, also known as PECAM-1 (Platelet Endothelial Cell Adhesion Molecule-1). CD31 is a 130 kDa integral membrane protein, a member of the immunoglobulin superfamily, that mediates cell-to-cell adhesion. CD31 is expressed constitutively on the surface of adult and embryonic endothelial cells and is weakly expressed on many peripheral leukocytes and platelets. It has also been detected on bone marrow-derived hematopoietic stem cells and embryonic stem cells. CD31 is involved in the transendothelial emigration of neutrophils, and neutrophil PECAM-1 appears to be down-regulated after extravasation into inflamed tissues. Multiple alternatively spliced isoforms are detected during early post-implantation embryonic development, this alternative splicing is involved in the regulation of ligand specificity. CD38 and vitronectin receptor (alphavbeta3 integrin, CD51/CD61) are proposed to be ligands for CD31. CD31-mediated endothelial cell-cell interactions are involved in angiogenesis. The MEC13.3 mAb inhibits a variety of in vitro and in vivo functions mediated by CD31.
Synonyms: PECAM-1

Pathways: Regulation of Actin Filament Polymerization