Interferon gamma antibody

Catalog No. ABIN1176896

Product Details anti-Interferon gamma Antibody

Target: Interferon gamma (IFNG)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Interferon gamma antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Neutralization (Neut), Blocking Reagent (BR), Intracellular Staining (ICS)

Brand: BD Pharmingen™

Characteristics: Neutralization Activity: This antibody has been reported to be useful for the neutralization of human IFN-gamma anti-viral activity. Neutralization activity may be measured with a encphalomyocarditis viral (EMCV) resistance assay using 1 ng/mL recombinant human IFN-gamma (Cat. No. 554616) with A549 indicator cells.
50% Neutralization (ND50) at 2 - 50 ng/mL
>95% Neutralization at 100 - 300 ng/mL

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Human IFN-gamma Recombinant Protein

Clone: B27

Isotype: IgG1 kappa

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-Interferon gamma antibody (ABIN1176896)

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).

Favre, Wijdenes, Cabrillat, Djossou, Banchereau, de Vries: "Epitope mapping of recombinant human gamma interferon using monoclonal antibodies." in: Molecular immunology, Vol. 26, Issue 1, pp. 17-25, (1989) (PubMed).

Target Details for Interferon gamma

Target: Interferon gamma (IFNG)

Alternative Name: IFN-gamma (IFNG Products)

Synonyms: IFG antibody, IFI antibody, IFN-g antibody, Ifg antibody, IFNG2 antibody, IFN-gamma antibody, IFN-G antibody, IFNG antibody, IFNgamma antibody, TCRalpha antibody, INF-G antibody, ifng antibody, interferon gamma antibody, interferon, gamma 1-2 antibody, IFNG antibody, Ifng antibody, ifng1-2 antibody

Background: The B27 antibody reacts with human interferon-gamma (IFN-gamma). This is a neutralizing antibody. The use of B27 antibody for epitope mapping of human IFN-gamma has been described. The B27 antibody has been reported not to bind to denatured IFN-gamma.

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy