Goat anti-Human IgG (F(ab')2 Region) Antibody (PE) - Preadsorbed

Catalog No. ABIN117695

Product Details

Target: IgG

Binding Specificity: F(ab')2 Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: PE

Application: Flow Cytometry (FACS), Immunomicroscopy (IM)

Purification: Preadsorption: Solid phase absorption

Immunogen: Human IgG F(ab')2 fragment.

Application Details

Application Notes: Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as otherantibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10^6 cells in Flow cytometry isapproximately 1.0 g of antibody conjugate. Lesser amounts of reagent may be sufficientfor staining. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.

Comment:

Absorption / Emission: 490 nm, 545 nm and 565 nm / 580 nm

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative and 10 mg/mL Bovine Serum Albumin (BSA), IgG and Protease free, as stabilizer.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: DO NOT FREEZE! Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
Dilute only prior to immediate use

Storage: 4 °C

Storage Comment: This antibody is stable at 2-8 °C as an undiluted liquid.

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Synonyms: Human Immunoglobulin G