PDCD1LG2 antibody

Catalog No. ABIN1176961

Product Details anti-PDCD1LG2 Antibody

Target: PDCD1LG2 (Programmed Cell Death 1 Ligand 2 (PDCD1LG2))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This PDCD1LG2 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Mouse B7-DC

Clone: TY25

Isotype: IgG2a kappa

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-PDCD1LG2 antibody (ABIN1176961)

Shin, Kennedy, Gorski, Tsuchiya, Koseki, Azuma, Yagita, Chen, Powell, Pardoll, Housseau: "Cooperative B7-1/2 (CD80/CD86) and B7-DC costimulation of CD4+ T cells independent of the PD-1 receptor." in: The Journal of experimental medicine, Vol. 198, Issue 1, pp. 31-8, (2003) (PubMed).

Nguyen, Radhakrishnan, Ciric, Tamada, Shin, Pardoll, Chen, Rodriguez, Pease: "Cross-linking the B7 family molecule B7-DC directly activates immune functions of dendritic cells." in: The Journal of experimental medicine, Vol. 196, Issue 10, pp. 1393-8, (2002) (PubMed).

Latchman, Wood, Chernova, Chaudhary, Borde, Chernova, Iwai, Long, Brown, Nunes, Greenfield, Bourque, Boussiotis, Carter, Carreno, Malenkovich, Nishimura, Okazaki, Honjo, Sharpe, Freeman: "PD-L2 is a second ligand for PD-1 and inhibits T cell activation." in: Nature immunology, Vol. 2, Issue 3, pp. 261-8, (2001) (PubMed).

Target Details for PDCD1LG2

Target: PDCD1LG2 (Programmed Cell Death 1 Ligand 2 (PDCD1LG2))

Alternative Name: CD273 (PDCD1LG2 Products)

Synonyms: PDL2 antibody, B7DC antibody, Btdc antibody, CD273 antibody, PD-L2 antibody, PDCD1L2 antibody, bA574F11.2 antibody, B7-DC antibody, F730015O22Rik antibody, programmed cell death 1 ligand 2 antibody, PDCD1LG2 antibody, Pdcd1lg2 antibody

Background: The TY25 monoclonal antibody specifically binds to CD273, also known as B7-DC or PD-L2. CD273 is a 42-kDa type I membrane glycoprotein encoded by the Pdcd1lg2 gene of the B7 family of the Ig superfamily. Pdcd1lg2 mRNA is expressed selectively in dendritic cells (DC), liver, and a few transformed cell lines (hepatic, neuroblastoma, and myeloid), but not in lymphoid tissues or macrophages. The protein has not been detected on resting peripheral T and B lymphocytes, macrophages, or DC, but it is upregulated upon activation of T cells, macrophages, and DC by a variety of stimulatory factors. B7-DC's receptor, PD-1, contains an ITIM (Immunoreceptor Tyrosine-based Inhibitory Motif) on its intracytoplasmic region and is expressed on activated B and T lymphocytes, suggesting that B7-DC-PD-1 interaction may be involved in the negative regulation of immune responses. The second PD-1 ligand, B7-H1 (PD-L1), is also a member of the B7 family of the Ig superfamily. B7-DC may also participate in positive immunoregulation, or costimulation of T cells, through an additional receptor, which is not PD-1 and distinct from the alternate receptor for B7-H1. Moreover, B7-DC is the first B7 ligand that has been found to participate in bi-directional communication, ligation of B7-DC on DC stimulates those DC. The TY25 antibody blocks the binding of B7-DC to PD-1, but not to its alternate ligand.
Synonyms: Pdcd1lg2, B7dc, B7-DC, PD-1 ligand 2, PD-L2, Btdc