CD32/CD16 antibody

Catalog No. ABIN1176962

Product Details anti-CD32/CD16 Antibody

Target: CD32/CD16

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD32/CD16 antibody is un-conjugated

Application: Flow Cytometry (FACS), Blocking Reagent (BR)

Brand: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Mouse BALB/c Macrophage J774

Clone: 2-4G2

Isotype: IgG2b kappa

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-CD32/CD16 antibody (ABIN1176962)

Araujo-Jorge, Rivera, el Bouhdidi, Daëron, Carlier: "An Fc gamma RII-, Fc gamma RIII-specific monoclonal antibody (2.4G2) decreases acute Trypanosoma cruzi infection in mice." in: Infection and immunity, Vol. 61, Issue 11, pp. 4925-8, (1993) (PubMed).

Latour, Bonnerot, Fridman, Daëron: "Induction of tumor necrosis factor-alpha production by mast cells via Fc gamma R. Role of the Fc gamma RIII gamma subunit." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 6, pp. 2155-62, (1992) (PubMed).

Maeda, Burton, Padgett, Conrad, Huff, Masuda, Szakal, Tew: "Murine follicular dendritic cells and low affinity Fc receptors for IgE (Fc epsilon RII)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 8, pp. 2340-7, (1992) (PubMed).

Rodewald, Moingeon, Lucich, Dosiou, Lopez, Reinherz: "A population of early fetal thymocytes expressing Fc gamma RII/III contains precursors of T lymphocytes and natural killer cells." in: Cell, Vol. 69, Issue 1, pp. 139-50, (1992) (PubMed).

Ravetch, Luster, Weinshank, Kochan, Pavlovec, Portnoy, Hulmes, Pan, Unkeless: "Structural heterogeneity and functional domains of murine immunoglobulin G Fc receptors." in: Science (New York, N.Y.), Vol. 234, Issue 4777, pp. 718-25, (1986) (PubMed).

Titus, Finkelman, Stephany, Jones, Segal: "Quantitative analysis of Fc gamma receptors on murine spleen cell populations by using dual parameter flow cytometry." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 133, Issue 2, pp. 556-61, (1984) (PubMed).

Mellman, Unkeless: "Purificaton of a functional mouse Fc receptor through the use of a monoclonal antibody." in: The Journal of experimental medicine, Vol. 152, Issue 4, pp. 1048-69, (1980) (PubMed).

Unkeless: "Characterization of a monoclonal antibody directed against mouse macrophage and lymphocyte Fc receptors." in: The Journal of experimental medicine, Vol. 150, Issue 3, pp. 580-96, (1979) (PubMed).

Target Details for CD32/CD16

Target: CD32/CD16

Alternative Name: CD16/CD32 (CD32/CD16 Products)

Background: The 2.4G2 antibody reacts specifically with a common nonpolymorphic epitope on the extracellular domains of the mouse FcgammaIII and FcgammaII receptors. It has also been reported to bind the FcgammaI receptor (CD64) via its Fc domain. 2.4G2 mAb blocks non-antigen-specific binding of immunoglobulins to the FcgammaIII and FcgammaII, and possibly FcgammaI, receptors in vitro and in vivo. CD16 and/or CD32 are expressed on natural killer cells, monocytes, macrophages, dendritic cells (at low levels), Kupffer cells, granulocytes, mast cells, B lymphocytes, immature thymocytes, and some activated mature T lymphocytes.
Synonyms: FcgammaRIII/FcgammaRII, Fcgr3/Fcgr2