TLR4 /MD2 Complex antibody

Catalog No. ABIN1176997

Product Details

Target: TLR4 /MD2 Complex

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Brand: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Mouse Pro-B cell line Ba/F3 expressing transfected BALB/c mouse TLR4 and MD-2

Clone: MTS510

Isotype: IgG2a kappa

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References

Akashi, Saitoh, Wakabayashi, Kikuchi, Takamura, Nagai, Kusumoto, Fukase, Kusumoto, Adachi, Kosugi, Miyake: "Lipopolysaccharide interaction with cell surface Toll-like receptor 4-MD-2: higher affinity than that with MD-2 or CD14." in: The Journal of experimental medicine, Vol. 198, Issue 7, pp. 1035-42, (2003) (PubMed).

Kawasaki, Nogawa, Nishijima et al.: "Identification of mouse MD-2 residues important for forming the cell surface TLR4-MD-2 complex recognized by anti-TLR4-MD-2 antibodies, and for conferring LPS and taxol responsiveness on mouse TLR4 ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 1, pp. 413-20, (2002) (PubMed).

Beutler, Poltorak: "The sole gateway to endotoxin response: how LPS was identified as Tlr4, and its role in innate immunity." in: Drug metabolism and disposition: the biological fate of chemicals, Vol. 29, Issue 4 Pt 2, pp. 474-8, (2001) (PubMed).

Akashi, Shimazu, Ogata, Nagai, Takeda, Kimoto, Miyake: "Cutting edge: cell surface expression and lipopolysaccharide signaling via the toll-like receptor 4-MD-2 complex on mouse peritoneal macrophages." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 7, pp. 3471-5, (2000) (PubMed).

Nomura, Akashi, Sakao, Sato, Kawai, Matsumoto, Nakanishi, Kimoto, Miyake, Takeda, Akira: "Cutting edge: endotoxin tolerance in mouse peritoneal macrophages correlates with down-regulation of surface toll-like receptor 4 expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 7, pp. 3476-9, (2000) (PubMed).

Shimazu, Akashi, Ogata, Nagai, Fukudome, Miyake, Kimoto: "MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4." in: The Journal of experimental medicine, Vol. 189, Issue 11, pp. 1777-82, (1999) (PubMed).

Target Details

Target: TLR4 /MD2 Complex

Alternative Name: CD284/MD-2 Complex

Background: The MTS510 antibody reacts with the molecular complex of Toll-Like Receptor 4 and MD-2 (TLR4-MD-2) which is expressed on LPS responsive macrophages. TLR4, a member of the Toll-Like Receptor Family, has been renamed CD284 and identified to be the transmembrane signal-transducing portion of the receptor for LPS. TLR4 associates on the cell surface with CD14 and MD-2, a 0.7 kDa molecule which is anchored to the membrane via its physical association with TLR4. The association of MD-2 with TLR4 is required for recognition of LPS and the anti-mitotic compound Taxol, which mimics the action of LPS on mouse cells. MTS510 mAb detects TLR4-MD-2 on the surface of thioglycollate-elicited macrophages from all mouse strains tested (ie, BALB/c, C57BL/6, C3H/HeJ, C3H/HeN, and DBA/1), including the C3H/HeJ strain which expresses an LPS-resistant mutant TLR4. Expression of TLR4-MD-2 is down-regulated on peritoneal macrophages after exposure to LPS, correlating with the occurrence of LPS tolerance. TLR4-MD-2 is not detected on splenocytes or thymocytes.
Synonyms: TLR4/MD-2 Complex