EIF4E antibody (pSer209) (PE)

Catalog No. ABIN1177052

Product Details anti-EIF4E Antibody (PE)

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Binding Specificity: pSer209

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This EIF4E antibody is conjugated to PE

Application: Intracellular Staining (ICS)

Brand: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human eIF4E Peptide

Clone: J77-925

Isotype: IgG1 kappa

Application Details

Application Notes: Either BD Cytofix™ fixation buffer or BD™ Phosflow Fix Buffer I may be used for cell fixation. Any of the three BD™ Phosflow permeabilization buffers may be used.

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-EIF4E antibody (PE) (ABIN1177052)

Culjkovic, Topisirovic, Borden: "Controlling gene expression through RNA regulons: the role of the eukaryotic translation initiation factor eIF4E." in: Cell cycle (Georgetown, Tex.), Vol. 6, Issue 1, pp. 65-9, (2007) (PubMed).

Wendel, Silva, Malina, Mills, Zhu, Ueda, Watanabe-Fukunaga, Fukunaga, Teruya-Feldstein, Pelletier, Lowe: "Dissecting eIF4E action in tumorigenesis." in: Genes & development, Vol. 21, Issue 24, pp. 3232-7, (2007) (PubMed).

Grolleau, Kaplan, Hanash, Beretta, Richardson: "Impaired translational response and increased protein kinase PKR expression in T cells from lupus patients." in: The Journal of clinical investigation, Vol. 106, Issue 12, pp. 1561-8, (2001) (PubMed).

Waskiewicz, Flynn, Proud, Cooper: "Mitogen-activated protein kinases activate the serine/threonine kinases Mnk1 and Mnk2." in: The EMBO journal, Vol. 16, Issue 8, pp. 1909-20, (1997) (PubMed).

Target Details for EIF4E

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Abstract: EIF4E Products

Synonyms: AUTS19 antibody, CBP antibody, EIF4E1 antibody, EIF4EL1 antibody, EIF4F antibody, eif4e-1 antibody, zgc:86680 antibody, EG668879 antibody, Eif4e-ps antibody, If4e antibody, eIF-4E antibody, 0260/09 antibody, 0587/11 antibody, 0589/11 antibody, 0919/12 antibody, 1004/13 antibody, 2 antibody, 7238 antibody, CG4035 antibody, CT13384 antibody, CT39424 antibody, CT39426 antibody, D-eIF4E antibody, Dmel\\CG4035 antibody, Eif4E antibody, Eif4e antibody, IF4E antibody, dEif4e antibody, deIF-4E antibody, deIF4E antibody, eIF 4E antibody, eIF-4E1 antibody, eIF-4E2 antibody, eIF-4EII antibody, eIF-4e antibody, eIF4E antibody, eIF4E-1 antibody, eIF4E-1/2 antibody, eIF4E-2 antibody, eIF4E1 antibody, eIF4EI antibody, eIF4e antibody, eif-4E antibody, eif-4e antibody, eif4e antibody, l(3)07238 antibody, l(3)67Af antibody, l(3)S025007 antibody, l(3)S026009 antibody, l(3)S058711 antibody, l(3)S091912 antibody, cbp antibody, eif4f antibody, eif4e1 antibody, eif4el1 antibody, EIF-4E antibody, zgc:101581 antibody, eukaryotic translation initiation factor 4E antibody, eukaryotic translation initiation factor 4ea antibody, Eukaryotic initiation factor 4E antibody, eucaryotic initiation factor 6 antibody, eukaryotic translation initiation factor 4E L homeolog antibody, eukaryotic translation initiation factor 4E family member 1B S homeolog antibody, translation initiation factor eIF4E antibody, eukaryotic translation initiation factor 4eb antibody, EIF4E antibody, eif4ea antibody, Eif4e antibody, eIF-4E antibody, eif6 antibody, eif4e antibody, eif4e.L antibody, eif4e1b.S antibody, LOC100533326 antibody, eif4eb antibody

Background: The eukaryotic translation Initiation Factor 4E (eIF4E) is a 25-kDa phosphoprotein that specifically binds to the 7-methylguanosine-containing cap of mRNA. eIF4E is the rate-limiting component for the initiation of cap-dependent translation by the eIF4F translation initiation complex, which is composed of eIF4E, eIF4G, and eIF4A. This complex promotes the unwinding of secondary structure at the 5' untranslated region of mRNA that is necessary to expose and locate the AUG initiation codon. Other functions of eIF4E have been identified, such as promoting the export of mRNAs that are involved in cell cycle progression from the nucleus and differentially regulating the translation of certain mRNAs in the cytoplasm. Three mechanisms for eIF4E regulation have been identified: Mnk1-mediated phosphorylation on serine 209 (S209) is required for eIF4E binding to the cap structure, over-expression of phosphorylated eIF4E can lead to increased cell proliferation, suppression of apoptosis, and a transformed phenotype, and interactions with nonphosphorylated eIF4E-binding proteins inhibit the formation of the eIF4F complex. The J77-925 monoclonal antibody recognizes the phosphorylated S209 (pS209) of eIF4E.
Synonyms: eIF-4E, CBP, EIF4E1, EIF4EL1, EIF4F, mRNA cap-binding protein

Pathways: BCR Signaling