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Goat anti-Human IgM (Fc5mu Region) Antibody (FITC) - Preadsorbed

FACS, IM Polyclonal FITC
Catalog No. ABIN117707
  • Target See all IgM products
    IgM
    Binding Specificity
    • 132
    • 110
    • 86
    • 41
    • 16
    • 8
    • 7
    • 1
    • 1
    Fc5mu Region
    Reactivity
    • 185
    • 129
    • 65
    • 37
    • 30
    • 23
    • 22
    • 19
    • 14
    • 13
    • 13
    • 13
    • 13
    • 12
    • 11
    • 4
    • 2
    Human
    Host
    • 312
    • 176
    • 73
    • 15
    • 13
    • 5
    • 4
    • 1
    Goat
    Clonality
    • 484
    • 78
    Polyclonal
    Conjugate
    • 109
    • 76
    • 76
    • 55
    • 32
    • 25
    • 24
    • 15
    • 12
    • 11
    • 11
    • 11
    • 10
    • 9
    • 9
    • 9
    • 7
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    FITC
    Application
    • 312
    • 236
    • 228
    • 187
    • 143
    • 128
    • 64
    • 62
    • 47
    • 47
    • 46
    • 42
    • 38
    • 28
    • 20
    • 18
    • 13
    • 6
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunomicroscopy (IM)
    Characteristics
    Molar Ratio: 2.7 moles FITC per mole of Goat IgG F(ab')2.
    Purification
    Preadsorption: Solid phase absorption
    Immunogen
    Human IgM Fc5µ fragment.
  • Application Notes
    Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as otherantibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Flow Cytometry: 1:2,000-1:10,000. Immunofluorescence microscopy: 1:500-1:2,500.

    Other applications not tested.
    Otimal dilutions are dependent on conditions and should be determined by the user.
    Comment

    Absorption / Emission: 495 nm / 528 nm

    Restrictions
    For Research Use only
  • Reconstitution
    Restore with 1.5 mL of deionized water (or equivalent)
    Concentration
    0.75 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01 % (w/v) Sodium Azide as preservative.
    Preservative
    Sodium azide
    Precaution of Use
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Handling Advice
    Product is photosensitive and should be protected from light.
    Dilute only prior to immediate use
    Storage
    4 °C/-20 °C
    Storage Comment
    Store vial at 4 °C before opening. This product is stable at 4 °C as an undiluted liquid.
  • Target
    IgM
    Abstract
    IgM Products
    Target Type
    Antibody
    Background
    Synonyms: Human Immunoglobulin M
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