PLC-g2 antibody (Alexa Fluor 488)

Catalog No. ABIN1177148

Product Details

Target: PLC-g2

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: Alexa Fluor 488

Application: Intracellular Staining (ICS)

Brand: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human PLC-gamma2 Peptide

Clone: K86-1161

Isotype: IgG1 kappa

Application Details

Application Notes: This PLC-gamma2-specific antibody conjugate may be used with conjugates of anti-PLC-gamma2 (pY759) mAb K86-689.37 to distinguish the expression of total versus phosphorylated PLC-gamma2. This antibody conjugate is suitable for intracellular staining of human whole blood and mouse splenocytes using the BD Phosflow™ Lyse/Fix Buffer and the BD Phosflow™ Perm Buffer II.

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.

References

Kim, Sekiya, Poulin, Bae, Rhee: "Mechanism of B-cell receptor-induced phosphorylation and activation of phospholipase C-gamma2." in: Molecular and cellular biology, Vol. 24, Issue 22, pp. 9986-99, (2004) (PubMed).

Target Details

Target: PLC-g2

Background: The Phospholipase C (PLC) isozymes hydrolyze phosphatidyl inositol biphosphate to inositol triphosphate and diacylglycerol. The former causes release of calcium from the endoplasmic reticulum, while the latter is an activator of Protein Kinase C. Within the PLC family, PLC-g is the only member that contains SH2 and SH3 domains. These domains enable it to interact with receptor tyrosine kinases and become enzymatically activated via phosphorylation. It exists as two isoforms: 1) PLC-g1, which is ubiquitously expressed, and 2) PLCg2, found primarily in the lymphoid system. PLC-g is essential for growth factor-induced cell motility and mitogenesis. Overexpression of PLC-g is evident in several forms of cancer, and it has been identified as a key mediator of PDGF-dependent cellular transformation. Thus regulation of PLC-g activity by growth factors is involved in cell growth and transformation. Although the immunogen for generation of the K86-1161 monoclonal antibody was a phosphorylated peptide, peptide blocking studies demonstrated that the mAb recognizes PLC-g2 regardless of phosphorylation status. This antibody was raised to a unique region of PLC-gamma2 and is predicted not to crossreact with PLC-gamma1