Interferon gamma antibody
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- Target See all Interferon gamma (IFNG) Antibodies
- Interferon gamma (IFNG)
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Interferon gamma antibody is un-conjugated
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Application
- ELISA, Neutralization (Neut)
- Brand
- BD Pharmingen™
- Characteristics
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Neutralization Activity: This antibody has been reported to be useful for the neutralization of human IFN-gamma anti-viral activity. Neutralization activity may be measured with a encphalomyocarditis viral (EMCV) resistance assay using 1 ng/mL recombinant human IFN-gamma (Cat. No. 554617) with A549 indicator cells.
50% Neutralization (ND50) at 0.25 - 2.0 µg/mL
> 90% Neutralization at 5 - 50 myg/mL - Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
- Immunogen
- Recombinant human IFN-gamma
- Clone
- NIB42
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product IFNG Primary Antibody
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- Application Notes
- ELISA: Purified NIB42 antibody can be useful as a capture antibody in sandwich ELISA for measuring human IFN-gamma protein levels. Purified NIB42 antibody can be paired with biotinylated 4S.B3 (Cat. No. 554550) as the detection antibody along with recombinant human IFN-gamma (Cat. No. 554617) as the standard. Investigators are highly encouraged, when measuring human IFN-gamma in serum or plasma, to utilize BD OptEIA™ Human IFN-gamma ELISA Set (Cat. No. 555142) or BD OptEIA™ Human IFN-gamma ELISA Kit II (Cat. No. 550612).
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
- No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
- Preservative
- Azide free
- Storage
- 4 °C
- Storage Comment
- Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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Rapid, quantitative, semiautomated assay for virus-induced and immune human interferons." in: Journal of clinical microbiology, Vol. 12, Issue 3, pp. 433-8, (1981) (PubMed).
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Rapid, quantitative, semiautomated assay for virus-induced and immune human interferons." in: Journal of clinical microbiology, Vol. 12, Issue 3, pp. 433-8, (1981) (PubMed).
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- Target
- Interferon gamma (IFNG)
- Alternative Name
- IFN-gamma (IFNG Products)
- Synonyms
- IFG antibody, IFI antibody, IFN-g antibody, Ifg antibody, IFNG2 antibody, IFN-gamma antibody, IFN-G antibody, IFNG antibody, IFNgamma antibody, TCRalpha antibody, INF-G antibody, ifng antibody, interferon gamma antibody, interferon, gamma 1-2 antibody, IFNG antibody, Ifng antibody, ifng1-2 antibody
- Background
- The NIB42 antibody reacts with human interferon-gamma (IFN-gamma). The immunogen used to generate the NIB42 hybridoma was recombinant human IFN-gamma. This is a neutralizing antibody.
- Pathways
- Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy
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