CXCR4 antibody

Catalog No. ABIN1177332

Product Details anti-CXCR4 Antibody

Target: CXCR4 (Chemokine (C-X-C Motif) Receptor 4 (CXCR4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CXCR4 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Clone: 12G5

Isotype: IgG2a kappa

Application Details

Application Notes: Flow cytometry: Chemokine receptors are known to internalize during manipulation resulting in low frequency expression. Investigators are advised to perform immunophenotyping studies of chemokine receptors on freshly collected samples (<24 Hrs). Incubation with the antibody should be done at 4°C in the dark. Cellular manipulation, such as Ficoll separation, freezing, or exposure to cold temperatures prior to staining should be minimized and have been shown to cause a decrease in staining intensity and/or inconsistent results. Investigators should note that alternative staining procedures may be neccessary. A multiple-step staining procedure is strongly recommended, in some instances, to amplify immunofluorescent signals for the flow cytometric analysis of human CXCR4 expression. Investigators may find the Purified NA/LE Mouse Anti-Human CD184 antibody to be useful in conjunction with appropriate secondary and tertiary reagents for detecting low frequency expression, such as with Biotin Goat Anti-Mouse Ig (MN 553999) and PE Streptavidin (MN 554061).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-CXCR4 antibody (ABIN1177332)

Simmons, Wilkinson, Reeves, Dittmar, Beddows, Weber, Carnegie, Desselberger, Gray, Weiss, Clapham: "Primary, syncytium-inducing human immunodeficiency virus type 1 isolates are dual-tropic and most can use either Lestr or CCR5 as coreceptors for virus entry." in: Journal of virology, Vol. 70, Issue 12, pp. 8355-60, (1997) (PubMed).

Endres, Clapham, Marsh, Ahuja, Turner, McKnight, Thomas, Stoebenau-Haggarty, Choe, Vance, Wells, Power, Sutterwala, Doms, Landau, Hoxie: "CD4-independent infection by HIV-2 is mediated by fusin/CXCR4." in: Cell, Vol. 87, Issue 4, pp. 745-56, (1997) (PubMed).

Feng, Broder, Kennedy, Berger: "HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor." in: Science (New York, N.Y.), Vol. 272, Issue 5263, pp. 872-7, (1996) (PubMed).

Target Details for CXCR4

Target: CXCR4 (Chemokine (C-X-C Motif) Receptor 4 (CXCR4))

Alternative Name: CD184 (CXCR4 Products)

Synonyms: CD184 antibody, D2S201E antibody, FB22 antibody, HM89 antibody, HSY3RR antibody, LAP3 antibody, LCR1 antibody, LESTR antibody, NPY3R antibody, NPYR antibody, NPYRL antibody, NPYY3R antibody, WHIM antibody, Cmkar4 antibody, PB-CKR antibody, PBSF/SDF-1 antibody, Sdf1r antibody, CXC-R4-B antibody, CXCR-4-B antibody, cd184 antibody, cxcr4 antibody, fb22 antibody, hm89 antibody, hsy3rr antibody, lap3 antibody, lcr1 antibody, lestr antibody, npy3r antibody, npyr antibody, npyrl antibody, npyy3r antibody, xcxcr4 antibody, CXC-R4 antibody, CXCR-4 antibody, d2s201e antibody, whim antibody, CXC-R4-A antibody, CXCR-4-A antibody, xCXCR4 antibody, CXCR4 antibody, cb403 antibody, zgc:109863 antibody, cb824 antibody, C-X-C motif chemokine receptor 4 antibody, chemokine (C-X-C motif) receptor 4 antibody, C-X-C motif chemokine receptor 4 S homeolog antibody, C-X-C chemokine receptor type 4 antibody, C-X-C motif chemokine receptor 4 L homeolog antibody, chemokine (C-X-C motif), receptor 4b antibody, chemokine (C-X-C motif) receptor 4a antibody, CXCR4 antibody, Cxcr4 antibody, cxcr4.S antibody, cxcr4 antibody, LOC100049444 antibody, cxcr4.L antibody, cxcr4b antibody, cxcr4a antibody

Background: Reacts with fusin (CXCR4), a seven-transmembrane domain, G-protein-linked glycoprotein. Fusin is expressed on a wide variety of CD4+ and CD4- cells including those from spleen, brain and lung. It has been shown to function as a cofactor for infection with T-cell tropic strains of HIV-1 and also as a receptor for CD4-independent infection. 12G5 antibody has been reported to block CD4-independent infection by HIV-2 and CD4-dependent infection by some T-cell tropic isolates of HIV-1.
Synonyms: CXCR4 (Fusin)

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events