RBX1 antibody (C-Term)

Catalog No. ABIN117957

This anti-RBX1 antibody is a Rabbit Polyclonal antibody detecting RBX1 in WB, IP and EIA. Suitable for Human, Mouse, Zebrafish (Danio rerio) and C. elegans.

Quick Overview for RBX1 antibody (C-Term) (ABIN117957)

Target: RBX1 (Ring-Box 1, E3 Ubiquitin Protein Ligase (RBX1))

Reactivity: Human, Mouse, Zebrafish (Danio rerio), C. elegans

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RBX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Product Details anti-RBX1 Antibody

Binding Specificity: AA 94-108, C-Term

Sequence: Human ROC1 (108 aa 12.2 kDa) : 1 maaamdvdtp sgtnsgagkk rfevkkwnav alwawdivvd ncaicrnhim dlciecqanq 61 asatseectv awgvcnhafh fhcisrwlkt rqvcpldnre wefqkygh

Specificity: This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human, mouse, C.elgans and zebra fish ROC1. Cross reactivity may also occur with ROC1 from other sources. Sufficient sequence differences exist to suggest that this antibody would not react with other RING box proteins such as ROC2 and APC11.

Purification: Delipidation and defibrination.

Immunogen: Prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 94-108 of Human ROC1 (C-terminal) coupled to KLH.

Application Details

Application Notes: This antibody reacts with human ROC1 by Western blot (1: 500-1: 1,000) andImmunoprecipitation. The antibody immunoprecipitates in vitro translated protein andprotein from overexpressing cell lysates (using HeLa and NIH-3T3, and others). Coimmunoprecipitation of related cullin proteins does occur. Coimmunoprecipitation ofubiquitin ligase activity will also occur. A 12.2 kDa band corresponding to human ROC1 isdetected. Most cell lines expressing ROC1 can be used as a positive control.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 85 mg/mL (by Refractometry)

Storage: 4 °C

Storage Comment: Store undiluted at 2-8 °C.

Target Details for RBX1

Target: RBX1 (Ring-Box 1, E3 Ubiquitin Protein Ligase (RBX1))

Alternative Name: RBX1 / RNF75

Background: ROC1 also known as RING-box protein 1, Rbx1, Regulator of cullins 1, RING finger protein 75, and ZYP protein, is a component of the SCF (SKP1-CUL1-F-box protein) and the CBC(VHL) (CUL2-elonging BC-VHL) E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. ROC1 appears to recruit the E2 ubiquitination enzyme through the RING-type zinc finger in a manner similar to CDC34, and brings it into close proximity to the substrate. The RING-type zinc finger domain is essential for ubiquitin ligase activity. ROC1 probably also stimulates CDC34 autoubiquitination and promotes the neddylation of CUL1 and probably CUL2. ROC1 has a cytoplasmic and nuclear localization and is widely expressed in most tissues.Synonyms: E3 ubiquitin-protein ligase RBX1, Protein ZYP, RING finger protein 75, RING-box protein 1, ROC1, Regulator of cullins 1

Gene ID: 9978

NCBI Accession: NP_055063

UniProt: P62877

Pathways: Cell Division Cycle, M Phase, SARS-CoV-2 Protein Interactome