DNMT3L antibody (AA 152-164)

Catalog No. ABIN118012

Product Details anti-DNMT3L Antibody

Target: DNMT3L (TRDMT1) (DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1))

Binding Specificity: AA 152-164

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNMT3L antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Sequence: Peptide: G-L-L-Q-R-R-R-K-W-R-S-Q-L Protein: 1 maaipaldpe aepsmdvilv gsselsssvs pgtgrdliay evkanqrnie diciccgslq 61 vhtqhplfeg gicapckdkf ldalflyddd gyqsycsicc sgetllicgn pdctrcycfe 121 cvdslvgpgt sgkvhamsnw vcylclpssr sgllqrrrkw rsqlkafydr esenplemfe 181 tvpvwrrqpv rvlslfedik keltslgfle sgsdpgqlkh vvdvtdtvrk dveewgpfdl 241 vygatpplgh tcdrppswyl fqfhrllqya rpkpgspgpf fwmfvdnlvl nkedldvasr 301 flemepvtip dvhggslqna vrvwsnipai rsrhwalvse eelsllaqnk qssklaakwp 361 tklvkncflp lreyfkyfst eltssl

Specificity: This antibody reacts with DNMT3L.

Cross-Reactivity (Details): Species reactivity (expected):Mouse (76 %).
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography using the immunizing peptide after immobilization to a solid phase

Immunogen: A synthetic peptide corresponding to aa 152-164 of Human DNMT3L.

Application Details

Application Notes: This product has been assayed by ELISA, Western blotting and Immunoprecipitation. The antibody was tested by ELISA against 0.1 μg of the immunizing peptide. A1/1,000-1/3,000 dilution of the antibody is recommended for this assay. For Western blotting, first Immunoprecipitate (1/100 dilution) the antigen using this

Restrictions: For Research Use only

Handling

Concentration: 1.43 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, containing 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Shelf live: One year from despatch.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Should this product contain a precipitate we recommend microcentrifugation before use.

Target Details for DNMT3L

Target: DNMT3L (TRDMT1) (DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1))

Alternative Name: DNMT3L (TRDMT1 Products)

Synonyms: DNMT3L antibody, D6Ertd14e antibody, ecat7 antibody, DNA methyltransferase 3 like antibody, DNA (cytosine-5-)-methyltransferase 3-like antibody, DNMT3L antibody, Dnmt3l antibody

Background: DNMT 3L is a nuclear protein that has similarity to DNA methyltransferases, involved in de novo methylation of CpG islands. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1.Synonyms: DNA (cytosine-5)-methyltransferase 3-like

Gene ID: 29947

NCBI Accession: NP_037501

UniProt: Q9UJW3