Mouse anti-Rat IgG1 (Chain gamma), (Heavy Chain) Antibody (FITC)

Catalog No. ABIN118434

Product Details

Target: IgG1

Binding Specificity: Chain gamma, Heavy Chain

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: FITC

Application: Flow Cytometry (FACS)

Purification: Affinity Chromatography

Immunogen: Rat IR27 IgG1 myeloma. Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.

Clone: MARG1-2

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: 5 μg /mL, use 50 μL of the suggested working dilution to label 10^6 cells in 100 μL.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.

Comment:

Excitation/Emission wavelength: 494 nm/514 nm

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide and 50 % Glycerol

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.

Target Details

Target: IgG1

Abstract: IgG1 Products

Target Type: Antibody

Background: Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions, to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 10e11 variants. Immunoglobulins, or antibodies, are a complex, heterogeneous mixture of proteins that exhibit two fundamental types of structural variation. Subtle structural differences in their antigen combining sites, or variable regions, account for their unique antigen binding specificities.Synonyms: Rat Immunoglobulin G1

UniProt: P20759