Mouse anti-Rat IgG2a Antibody (FITC)
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- Target See all IgG2a products
- IgG2a
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Reactivity
- Rat
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- FITC
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Application
- Flow Cytometry (FACS)
- Purification
- Affinity Chromatography
- Immunogen
- Purified Rat immunoglobulins from the IR33 - anti horse Ferritin antibody. Spleen cells from immunised BALB/c mice were fused with cells of the PAI-0 mouse myeloma cell line.
- Clone
- MARG2a-1
- Isotype
- IgG1
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- Application Notes
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Flow Cytometry: 5 μg/mL, use 50 μL of the suggested working dilution to label 10^6 cells in 100 μL.
Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user. - Comment
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Excitation/Emission wavelength: 494 nm/514 nm
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- PBS containing 0.09 % Sodium Azide and 50 % Glycerol
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
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Product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing. - Storage
- 4 °C/-20 °C
- Storage Comment
- Store undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
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- Target
- IgG2a
- Abstract
- IgG2a Products
- Target Type
- Antibody
- Background
- Synonyms: Rat Immunoglobulin G2a
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