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CD3 (CD3E) antibody (FITC)

Antigen

CD3 (CD3E)
Synonyms T3E, TCRE, FLJ18683
Clonality Monoclonal (UCHT1)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human, Primate
Conjugate
Application
Alternatives Flow Cytometry (FACS)
2 references available
Catalog no. ABIN118478
Quantity 0.1 mg  (0.1mg/ml)
Price 385.00 $   Plus shipping costs $45.00
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anti-CD3 (CD3E) antibody (FITC)

Additional Information
Application Details Publications Images Alternatives back to top

Alternative name CD3
UniProt P07766
Immunogen Human infant thymocytes and lymphocytes from a patient with Sezary Syndrome. Spleen cells from immunised BALB/c mice were fused with cells of the P3/NS1/1-Ag4-1 mouse myeloma cell line
Cross-Reactivity Human, Primate
Isotype IgG1
Clone UCHT1
Description CD3 is a surface protein of peripheral blood T cells of molecular weight 19 kD. The pan T cell marker is present on more than 95% of circulating human peripheral T cells. Alternate Names: CD3D, CD3E, CD3G, CD3Z, CD3 delta chain, CD3 epsilon chain, CD3 gamma chain, CD3 zeta chain, T-cell surface glycoprotein CD3, T3D, T3G, CD247, T3Z, TCRZ.

Application Details
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Application Notes Flow Cytometry: Use 10 ul of 1/10 diluted antibody to label 10e6 cells or 100ul of lysed whole peripheral blood. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Concentration 0.1mg/ml
Buffer PBS containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light. Shelf Life: one year from despatch.
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells
Restrictions For Research Use only

Publications
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Beverley, Callard: "Distinctive functional characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody." in: European journal of immunology, Vol. 11, Issue 4, pp. 329-34, 1981 (PubMed).

Zarkesh-Esfahani, Pockley, Metcalfe et al.: "High-dose leptin activates human leukocytes via receptor expression on monocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 8, pp. 4593-9, 2001 (PubMed).

Alternatives
Application Details Publications Images back to top

Alternatives for antigen "CD3 (CD3E)", type "Antibodies"
Hosts Mouse (305), Rabbit (65), Rat (52), Hamster (39)
Reactivities Human (318), Mouse (Murine) (112), Rat (Rattus) (44), Primate (20), Dog (Canine) (10), Monkey (4), Cat (Feline) (1), Horse (Equine) (1), Rhesus Monkey (1)
Applications Flow Cytometry (FACS) (321), Immunoprecipitation (IP) (81), Functional Studies (Func) (72), Immunohistochemistry (Frozen Sections) (IHC (fro)) (63), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (53), Western Blotting (WB) (48), Immunofluorescence (IF) (37), Immunohistochemistry (Fixed) (IHC (fx)) (33), ELISA (28), Immunohistochemistry (IHC) (15), Immunocytochemistry (ICC) (12), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (7), Immunostaining (ISt) (4), Activation (Act) (3), Blocking Antibody (Inhibition) (3), Blocking Antibody (Inhibition) (2), Cytotoxicity Test (CyTox) (1), Dot Blot (DB) (1), Fluorescence Microscopy (FM) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates FITC (74), Biotin (46), PE (38), APC (26), RPE (7), Cy5 (6), PerCP (5), Alexa Fluor 488 (4), Cy5,PE (4), PE-Dyomics 647 (4), PE,Cy7 (3), RPE,Cy5 (3), SPRD (3), APC,Cy7 (2), Alexa Fluor 647 (2), Cy5.5 (2), Cy7,PE (2), Dyomics 647 (2), PE,Cy5.5 (2), PE-Cy5 (2), PE-Dyomics 590 (2), PerCP-Cy5.5 (2), Alexa Fluor 350 (1), Alexa Fluor 555 (1), CF405M (1), Cy3 (1), Cy7 (1), Gold (1), HRP (1), Orange Cytognos 515 (1), PE,Cy3 (1), PE,Cy5 (1), PE,Dyomics 647 (1), RPE,Cy7 (1)
Epitopes Chain epsilon (6), AA 156-168 (3), Activation Site (2), Internal Region (1)