IL-5 antibody

Catalog No. ABIN118519

This Rat Monoclonal antibody specifically detects IL-5 in WB, FACS and EIA. It exhibits reactivity toward Human, Mouse and Guinea Pig.

Quick Overview for IL-5 antibody (ABIN118519)

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Reactivity: Human, Mouse, Guinea Pig

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-5 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Enzyme Immunoassay (EIA)

Clone: TRFK5

Product Details anti-IL-5 Antibody

Specificity: This antibody is specific for Interleukin 5 (IL-5). Clone TRFK5 may be used to neutralise IL-5 bioactivity in a functional assay (1,2).

Purification: Affinity chromatography on Protein G

Immunogen: Purified natural murine IL-5.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: Membrane permeabilisation is required for this application. ELISA: This antibody may be used in combination with clone TRFK4 (ABIN181863) in ELISA and ELISPOT assays. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for IL-5

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Alternative Name: Interleukin-5 / IL5

Background: IL-5 is a T cell derived haematopoietic growth factor, which stimulates eosinophil differentiation and activation.Synonyms: B-cell differentiation factor I, Eosinophil differentiation factor, IL-5, T-cell replacing factor, TRF

Gene ID: 3567

NCBI Accession: NP_000870

UniProt: P05113

Pathways: JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour