CD28 antibody

Catalog No. ABIN118582

Product Details anti-CD28 Antibody

Target: CD28

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This CD28 antibody is un-conjugated

Application: Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody recognises the CD28 cell surface antigen, a 44 kDa glycoprotein expressed by T lymphocytes. The antibody enhances the MLR response. It has been reported that this clone also recognises an epitope of CD28 expressed by NK cells, which is not recognised by clones of 9.3 or CD28.2.

Purification: Affinity chromatography on Protein G

Endotoxin Level: Low Endotoxin

Immunogen: Human peripheral blood T-cells. Spleen cells from an immunised DA rat were fused with cells of the Y3/Ag 1.2.3 rat myeloma cell line.

Clone: YTH913-12

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry: 1/50 - 1/100, Use 10 mLof the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections. Functional Assays: Stimulation of MLR response.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.01 EU/ug

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for CD28

Target: CD28

Alternative Name: CD28 (CD28 Products)

Synonyms: Tp44 antibody, CD28RNA antibody, cd28 antibody, CD28 molecule antibody, CD28 antigen antibody, Cd28 molecule antibody, CD28 molecule L homeolog antibody, CD28 antibody, Cd28 antibody, cd28.L antibody

Background: Human CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. It is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes (approximately 95 % of CD4+ cells and 50 % of CD8+ cells). Mature thymocytes exhibit higher levels of CD28 than the immature cells. Activation of T cells results in enhanced CD28 expression. T cell activation requires two combined signals provided by antigen presenting cells. The first is mediated via the T cell receptor following its interaction with antigenic peptide MHC complexes, and the second is delivered by accessory or costimulating molecules through their counter receptors on T lymphocytes. CD28 bears structural homology to CTLA 4 which is expressed at very low levels on the surface of CD4+ and CD8+ peripheral blood cells only following activation. CD28 is the natural receptor for the B7/BB 1 ligand (CD80) a 55-60 kDa glycoprotein which is expressed on activated B lymphocytes, on dendritic cells and on interferon gamma treated monocytes. The binding of B7 1/BB 1 Molecules to CD28 is involved in T lymphocyte activation and in the initiation and maintenance of chronic inflammation.Synonyms: T-cell-specific surface glycoprotein CD28, TP44

Gene ID: 940

NCBI Accession: NP_006130

UniProt: P10747

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response