CD34 antibody (CD34 Molecule)

Details for Product anti-CD34 Antibody No. ABIN118603, Supplier: Log in to see
Antigen
  • AU040960
  • CD34 antigen
  • CD34 molecule
  • LOC100343670
  • Cd34
  • CD34
Reactivity
Human, Monkey
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240
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Host
Mouse
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7
3
3
Clonality (Clone)
Monoclonal ()
Conjugate
This CD34 antibody is un-conjugated
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85
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2
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Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
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2
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Options
Supplier
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Immunogen Human endothelial cell membrane vesicles. Spleen cells from immunised NZB mice were fused with cells of the mouse NSO myeloma cell line.
Clone QBEND-10
Isotype IgG1
Specificity This antibody recognises the Class II epitope on CD34. Ultrastructure studies, using an indirect immunogold technique on ultra thin sections of human umbilical artery, have revealed that this antigen is associated to a degree with the basoluminal surfaces of endothelium, a slight reaction with an intracytoplasmic vesicular compartment is shown. Predominant staining is on the endothelial cell membrane. Some cross reactions with vascular associated adventitia and some basement membranes. Clone QBEND-10 immunoprecipitates a glycoprotein of Mr 110 kDa expressed on haemopoietic cells and on the established myeloid leukemic cell line KG1a.
Purification Affinity Chromatography on Protein A
Alternative Name CD34 (CD34 Antibody Abstract)
Background CD34 is a single chain transmembrane glycoprotein of 105 - 120kD. Expressed on haemopoietic cells, including bone marrow and blood. It is expressed on the majority of cells in acute lymphoid leukaemia (ALL) as well as cells in acute myelogenous leukaemia (AML). It is also found on capillary endothelial cells. There are three classes of CD34 epitopes defined by differential sensitivity to enzymatic cleavage with glycoprotease from Pasteurella haemolytica and with neuraminidase.Synonyms: Hematopoietic progenitor cell antigen CD34, Hematopoietic progenitor cell marker
Gene ID 947
NCBI Accession NP_001020280
UniProt P28906
Research Area Hematopoietic Progenitors, Hematopoietic Stem Cells, Cardiogenesis, Lineage Markers, CD Antigens, Surface Receptors of Immune Cells
Application Notes Western Blot. Immunoprecipitation. Flow Cytometry: Use 10 μL of 1/100-1/200 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections (1/500-1/1000): This product does not require antigen retrieval using heat treatment or protein digestion. However staining of paraffin embedded formalin fixed tissues may be enhanced by enzyme pre-treatment using pronase or heat treatment using citrate buffer. Recommended positive control: Tonsil, Bone Marrow.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS containing 0.09 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Yoshino, Ami, Terao, Tashiro, Honda: "Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies." in: Experimental animals / Japanese Association for Laboratory Animal Science, Vol. 49, Issue 2, pp. 97-110, 2000 (PubMed).

Sopper, Stahl-Hennig, Demuth, Johnston, Dörries, ter Meulen: "Lymphocyte subsets and expression of differentiation markers in blood and lymphoid organs of rhesus monkeys." in: Cytometry, Vol. 29, Issue 4, pp. 351-62, 1998 (PubMed).

Pammer, Plettenberg, Weninger, Diller, Mildner, Uthman, Issing, Stürzl, Tschachler: "CD40 antigen is expressed by endothelial cells and tumor cells in Kaposi's sarcoma." in: The American journal of pathology, Vol. 148, Issue 5, pp. 1387-96, 1996 (PubMed).

Fina, Molgaard, Robertson, Bradley, Monaghan, Delia, Sutherland, Baker, Greaves: "Expression of the CD34 gene in vascular endothelial cells." in: Blood, Vol. 75, Issue 12, pp. 2417-26, 1990 (PubMed).