Keratin 14 (KRT14) (C-Term) antibody (FITC)

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Antigen
  • Krt14
  • KRT14
  • CK14
  • ouro2
  • EBS3
  • EBS4
  • K14
  • NFJ
  • AI626930
  • Krt-1.14
  • Krt1-14
  • Ka14
  • keratin 14-like
  • keratin 14
  • keratin, type I cytoskeletal 10-like
  • Krt14l
  • KRT14
  • krt14
  • Krt14
  • LOC771977
Epitope
C-Term
125
27
6
5
5
4
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
296
137
129
18
14
9
5
3
2
2
1
1
1
1
1
Host
Mouse
184
109
5
4
Clonality (Clone)
Monoclonal ()
Conjugate
FITC
9
7
6
4
4
4
4
4
4
4
4
4
4
4
4
4
4
2
Application
Flow Cytometry (FACS), Immunofluorescence (IF)
194
148
120
96
87
83
80
53
19
6
6
4
3
1
Options
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Immunogen Last 15 C-terminal residues of Human cytokeratin 14 conjugated to thyroglobulin.
Clone LL002
Isotype IgG3
Specificity Recognizes Cytokeratin 14, a type I intermediate filament, expressed by stratifying epithelial cells and can be used to distinguish these cell types from simple epithelial cells, which do not express Cytokeratin 14. Mouse anti Cytokeratin 14, clone LL002 has been reported to be suitable for use in Western blotting (Alam et al. 2011).
Purification Affinity Chromatography on Protein A
Alternative Name Cytokeratin 14 (KRT14 Antibody Abstract)
Background Keratin 14 is a member of the keratin family, the most diverse group of intermediate filaments. It is usually found as a heterotetramer with two molecules of keratin 5, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. Keratin 14 has been studied as a prognostic marker in breast cancer.Synonyms: CK14, Cytokeratin-14, K14, KRT14, Keratin 14, Keratin Type I Cytoskeletal 14
Gene ID 3861
NCBI Accession NP_000517
UniProt P02533
Research Area Signaling, Cytoskeleton, Cytokeratins, Extracellular Matrix, Cancer
Application Notes Flow Cytometry: Use 10 μL of neat - 1/10 diluted antibody to label 10^6 cells in 100 μL (Requires Cell Permeabilisation).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.1 mg/mL
Buffer PBS, 0.09 % Sodium Azide, 1 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Abdeen, Salah, Maly, Smith, Tufail, Abu-Odeh, Zanesi, Croce, Nawaz, Aqeilan: "Wwox inactivation enhances mammary tumorigenesis." in: Oncogene, Vol. 30, Issue 36, pp. 3900-6, 2011 (PubMed).

Turner, Subramanian, Thomas, Hinley, Abbas, Stahlschmidt, Southgate: "Transplantation of autologous differentiated urothelium in an experimental model of composite cystoplasty." in: European urology, Vol. 59, Issue 3, pp. 447-54, 2011 (PubMed).

Matos, Faustino, Lopes, Rutteman, Gärtner: "Detection of lymph node micrometastases in malignant mammary tumours in dogs by cytokeratin immunostaining." in: The Veterinary record, Vol. 158, Issue 18, pp. 626-30, 2006 (PubMed).

Purkis, Steel, Mackenzie, Nathrath, Leigh, Lane: "Antibody markers of basal cells in complex epithelia." in: Journal of cell science, Vol. 97 ( Pt 1), pp. 39-50, 1991 (PubMed).

Lane, Alexander: "Use of keratin antibodies in tumor diagnosis." in: Seminars in cancer biology, Vol. 1, Issue 3, pp. 165-79, 1991 (PubMed).

Wetzels, Holland, van Haelst, Lane, Leigh, Ramaekers: "Detection of basement membrane components and basal cell keratin 14 in noninvasive and invasive carcinomas of the breast." in: The American journal of pathology, Vol. 134, Issue 3, pp. 571-9, 1989 (PubMed).

Moll, Franke, Schiller, Geiger, Krepler: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 (PubMed).