Toll-Like Receptor 4 (TLR4) antibody (PE)

Details for Product No. ABIN119333
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Antigen
Synonyms ARMD10, CD284, TLR-4, TOLL, Lps, Ly87, Ran/M1, Rasl2-8
Reactivity
Dog (Canine), Guinea Pig, Human, Monkey, Pig (Porcine)
(226), (71), (26), (19), (19), (16), (10), (8), (1), (1)
Host
Mouse
(147), (120), (9), (5)
Clonality (Clone)
Monoclonal ()
Conjugate
PE
(25), (23), (15), (5), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Flow Cytometry (FACS)
(153), (148), (67), (58), (52), (51), (36), (14), (14), (13), (10), (7), (3), (2), (1)
Pubmed 2 references available
Quantity 100 tests
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days
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Catalog No. ABIN119333
434.50 $
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Immunogen Ba/F3 cell line expressing TLR4 (CD284). Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Clone HTA125
Isotype IgG2a
Specificity This antibody recognises the Toll like receptor 4 (TLR4) cell surface antigen. This antibody has been demonstrated to block activation of monocytes with LPS. We recommend the use of SM1677LE for this purpose. Species Reactivity: Tested: Human, Rhesus Monkey, Guinea Pig, Porcine and Canine (Dog).
Purification Affinity Chromatography on Protein G
Alternative Name CD284 / TLR4
Background TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule. TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS.
Alternate names: Toll-like receptor 4
NCBI Accession NM_138554
UniProt O00206
Research Area Signaling, Immunology, Inflammation
Application Notes Flow Cytometry: use 10 ul of neat antibody to label 10e6 cells or 100 ul whole blood. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore with 1.0 mL distilled water.
Buffer PBS 0.09% Sodium Azide 1% BSA and 5% Sucrose
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Prior to and following reconstitution store the antibody at -20°C.
Expiry Date 12 months
Supplier Images
anti-Toll-Like Receptor 4 (TLR4) antibody (PE) Staining of U937 cells with mouse anti human CD284-PE (ABIN119333).
Background publications Shimazu, Akashi, Ogata et al.: "MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4." in: The Journal of experimental medicine, Vol. 189, Issue 11, pp. 1777-82, 1999 (PubMed).

General Jiang, Akashi, Miyake et al.: "Lipopolysaccharide induces physical proximity between CD14 and toll-like receptor 4 (TLR4) prior to nuclear translocation of NF-kappa B." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 7, pp. 3541-4, 2000 (PubMed).

Validation Images
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