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Details for Product No. ABIN119333

Toll-Like Receptor 4 (TLR4) antibody (PE)

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Antigen
Synonyms ARMD10, CD284, TLR-4, TOLL, Lps, Ly87, Ran/M1, Rasl2-8
Reactivity
»Alternatives Dog (Canine), Guinea Pig, Human, Monkey, Pig (Porcine)
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
Application
»Alternatives Flow Cytometry (FACS)
Pubmed 2 references available
Catalog no. ABIN119333
Quantity 100 tests
Price
423.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days
Immunogen Ba/F3 cell line expressing TLR4 (CD284). Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Clone HTA125
Isotype IgG2a
Specificity This antibody recognises the Toll like receptor 4 (TLR4) cell surface antigen. This antibody has been demonstrated to block activation of monocytes with LPS. We recommend the use of SM1677LE for this purpose. Species Reactivity: Tested: Human, Rhesus Monkey, Guinea Pig, Porcine and Canine (Dog).
Purification Affinity Chromatography on Protein G
Alternative Name CD284 / TLR4
Background TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule. TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS.
Alternate names: Toll-like receptor 4
NCBI Accession NM_138554.3
UniProt O00206
Research Area Signaling, Immunology, Inflammation
Application Notes Flow Cytometry: use 10 ul of neat antibody to label 10e6 cells or 100 ul whole blood. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore with 1.0 mL distilled water.
Buffer PBS 0.09% Sodium Azide 1% BSA and 5% Sucrose
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Prior to and following reconstitution store the antibody at -20°C.
Expiry Date 12 months
Background publications Shimazu, Akashi, Ogata et al.: "MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4." in: The Journal of experimental medicine, Vol. 189, Issue 11, pp. 1777-82, 1999 (PubMed).

Jiang, Akashi, Miyake et al.: "Lipopolysaccharide induces physical proximity between CD14 and toll-like receptor 4 (TLR4) prior to nuclear translocation of NF-kappa B." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 7, pp. 3541-4, 2000 (PubMed).

Alternatives for antigen "Toll-Like Receptor 4 (TLR4)", type "Antibodies"
Hosts (140), (127), (20), (5)
Reactivities (222), (89), (35), (24), (23), (21), (18), (11), (4), (3), (2), (2), (1), (1), (1)
Applications (149), (142), (64), (62), (43), (32), (29), (18), (17), (10), (9), (7), (6), (3), (2), (1), (1), (1), (1)
Conjugates (30), (28), (17), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (23), (13), (9), (9), (7), (7), (6), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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