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IL-4 antibody (Interleukin 4)

Details for Product anti-IL4 Antibody No. ABIN119373, Supplier: Log in to see
Antigen
  • IL4
  • IL-4
  • BCGF-1
  • BCGF1
  • BSF-1
  • BSF1
  • Il-4
  • Il4e12
  • IL2
Alternatives
anti-Human IL-4 antibody for Enzyme Immunoassay
Reactivity
Human
330
184
52
25
19
15
7
7
7
6
6
5
4
3
3
3
2
2
1
Host
Rat
247
199
153
8
7
2
Clonality (Clone)
Monoclonal ()
Conjugate
This IL-4 antibody is un-conjugated
70
39
28
23
15
8
7
6
4
4
4
2
2
1
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
341
303
187
84
56
31
28
27
24
23
20
20
19
18
10
6
6
5
3
3
3
3
2
2
2
2
1
1
1
1
1
Supplier
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Immunogen Purified CHO cell expressed recombinant human IL-4.
Clone MP4-25D2
Isotype IgG1
Specificity This antibody is specific for Interleukin - 4 (IL-4). Clone MP4-25D2 is reported to neutralise IL-4 activity. We recommend the use of SM1702A for this purpose.
Purification Affinity chromatography on Protein G
Alternative Name Interleukin-4 / IL4 (IL4 Antibody Abstract)
Background IL-4 is a 20kD cytokine which is expressed by a subpopulation of activated T cells and mast cells.Synonyms: B-cell stimulatory factor 1, BSF1, Binetrakin, IL-4, Lymphocyte stimulatory factor 1, Pitrakinra
Gene ID 3565
NCBI Accession NP_000580
UniProt P05112
Pathways JAK-STAT Signaling
Application Notes Flow Cytometry: 1/10 - 1/50, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL, Membrane permeabilisation is required for this application. ELISA. Western Blot: 1/500 - 1/1000. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Western Blotting (WB) image for anti-IL-4 antibody (Interleukin 4) (ABIN119373) Western blot analysis of human IL-4 recombinant protein probed with RAT ANTI HUMAN IN...
Background publications Bie, Yuan, Zhao et al.: "Method for estimating the axial intensity derivative in the TIE with higher order intensity derivatives and noise suppression." in: Optics express, Vol. 20, Issue 7, pp. 8186-91, 2012 (PubMed).

Patterson, Behbahani, Kabat et al.: "Leukemia inhibitory factor inhibits HIV-1 replication and is upregulated in placentae from nontransmitting women." in: The Journal of clinical investigation, Vol. 107, Issue 3, pp. 287-94, 2001 (PubMed).

Krohn, Preis, Prehn: "Staurosporine-induced apoptosis of cultured rat hippocampal neurons involves caspase-1-like proteases as upstream initiators and increased production of superoxide as a main downstream effector." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 18, Issue 20, pp. 8186-97, 1998 (PubMed).

Kubota, Miller, Imaoka et al.: "Oral immunization with simian immunodeficiency virus p55gag and cholera toxin elicits both mucosal IgA and systemic IgG immune responses in nonhuman primates." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 11, pp. 5321-9, 1997 (PubMed).

Carvalho, Bacellar, Brownell et al.: "Restoration of IFN-gamma production and lymphocyte proliferation in visceral leishmaniasis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 12, pp. 5949-56, 1994 (PubMed).

Abrams, Roncarolo, Yssel et al.: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992 (PubMed).