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HLA Class I A-2 alpha (HLAA2) antibody (FITC)

Details for Product No. ABIN119576, Supplier: Log in to see
Antigen
Reactivity
Human
3
Host
Mouse
3
Clonality (Clone)
Monoclonal ()
Conjugate
FITC
1
Application
Flow Cytometry (FACS)
3
2
2
1
Supplier
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Immunogen Papain solubilised HLA-A2. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Clone BB7-2
Isotype IgG2b
Specificity This antibody recognises the HLA-A2 histocompatability antigen. The epitope recognised by this antibody has been studied extensively and would appear to include the carboxy-terminus of the alpha-2 helix and a turn on one of the underlying beta strands.
Purification Affinity chromatography on Protein G
Alternative Name HLA Class I A-2 alpha / HLA-A2
Background Synonyms: A-2 alpha chain, HLA class I histocompatibility antigen, HLAA2, MHC class I antigen A-2
Gene ID 100507703
NCBI Accession XP_003119274
UniProt P01892
Application Notes Flow cytometry (Neat - 1/10): Use 10 μL of the suggested working dilution to label 10^6 cells or 100 μL whole blood, this antibody may be used for the flow cytometric detection of HLA-A2 expression.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.1 mg/mL
Buffer PBS containing 0.09 % Sodium Azide and 1 % Bovine Sodium Albumin
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
 image for anti-HLA Class I A-2 alpha (HLAA2) antibody (FITC) (ABIN119576) Staining of human peripheral blood monocytes with Mouse anti Human HLA-A2:FITC (ABIN1...
Background publications Harig, Witzens, Krackhardt, Trojan, Barrett, Broderick, Zauls, Gribben: "Induction of cytotoxic T-cell responses against immunoglobulin V region-derived peptides modified at human leukocyte antigen-A2 binding residues." in: Blood, Vol. 98, Issue 10, pp. 2999-3005, 2001 (PubMed).

Hogan, Brown: "Localization and characterization of serologic epitopes on HLA-A2." in: Human immunology, Vol. 33, Issue 3, pp. 185-92, 1992 (PubMed).

Parham, Brodsky: "Partial purification and some properties of BB7.2. A cytotoxic monoclonal antibody with specificity for HLA-A2 and a variant of HLA-A28." in: Human immunology, Vol. 3, Issue 4, pp. 277-99, 1982 (PubMed).