This antibody recognizes the CD229 cell surface antigen, a 100-120 kDa cell surface glycoprotein that is the homologue of murine Ly-9.
Purification
Affinity Chromatography on Protein G
Immunogen
Mouse pre-B cell line 300.19 transfected with CD229 cDNA. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line.
Flow cytometry: 1/50 - 1/100, use 10 μL of the suggested working dilution to label 10^6 cells. Immunoprecipitation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS containing 0.09 % Sodium Azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
CD229 is a member of the immunoglobulin superfamily and may have a function in cell signalling. The antigen is expressed at low levels by mature T and B lymphocytes and at higher levels by single-positive thymocytes. Expression at variable levels is also seen in some cell lines, such as Daudi, U937 and HUT78.Synonyms: Ly-9, Lymphocyte antigen 9, T-lymphocyte surface antigen Ly-9